Pre-madelentivirusexpress RFPfluorescentreporterunderaminimalpromoterinsertedwith4tandemrepeatsofestrogenreceptorresponseelement(ER-RE),fordetectingEstrogen-receptorregulated signalingpathwayactivity.
Thislentivirus alsoexpresstheGFP fluorescentselection/normalizationMarkerundertheconstitutiveRSVpromoter. See ProductManual (.pdf)fordetails.
Ifdesired,youcanalsousea pathway“ControlLentivirus”toestablishtheNo-responsecontrolprofiletoyourpathwaystimulus.Thecorresponding pathwaycontrollentivirusis:CAT#:Path-Ctr16.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS.
Cat#:LVP974-G