Pre-madeoptionalinduciblelentiviralparticlesforexpressinghumantarget:KCNN4(alternativename:IK1,IKCA1,KCA4,KCa3.1,SK4,hIKCa1,hKCa4,hSK4).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_002250.2.ParticlesalsocontainsaRFP-BlasticidindualselectionMarker.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter(Note:itcan alsousedasregularconstitutivehighexpressionwithoutanyinducationrequired).ARFP-BlasticidinfusionmarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.Seeproductmanualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlinserum-freemedium.
Cat#:LVP516