Pre-madelentivirusexpress FireflyLuciferase3 reporterunderaminimalpromoterinsertedwith8tandemrepeatsofGliresponsiveelement,fordetectingHedgehog signalingpathwayacitvity.
Thislentivirus alsoexpresstheGFP fluorescentselection/normalizationMarkerundertheconstitutiveRSVpromoter. See ProductManual fordetails(.pdf).
Ifdesired,youcanalsousea pathway“ControlLentivirus”toestablishtheNo-responsecontrolprofiletoyourpathwaystimulus.Thecorresponding pathwaycontrollentivirusis:CAT#:Path-Ctr17.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS.
Cat#:LVP947-G