Gentarget/anti miRNA (H1) with RFPPuromycin marker/AmiRNA H1RP/1 Ea

One anti-microRNAlentiviector(5.0ug)andits ready-to-useanti-miRNA lentivirus(0.5ml),and One negativecontrolanti-miRNAlenitvector(5.0ug)anditscontrol lentivirus(0.5ml).

Theanti-miRNAwasexpressedunderan optionalTetracyclineinducible humanH1promoter.Itcanbeusedasconstitutiveanti-miRNAexpressionwithoutanyinduction, orusedastetracyclineinducibleexpressionwhenthe Tet-repressorprotein ispresentinadvance.Theanti-miRNAlentivectoraslocontainscontainsa RFP-Puromycidin fusiondualMarkerunderRsvpromoter,whichallowstoselectthepositivecellsbyPuromycinantibioticselectionorby cellsortingvia RFP signal. See Anti-miRNAlentivirus manual formoredetailsabouttheanti-miRNAlentivector.

Orderwillbedeliveredwithin 7-10 businessdays.

Cat#: A-miRNAH1-RP,

Amounts: (1)0.5mlofpre-madespecificanti-miRNAlentiviruswithtiter at~1×107 IFU/ml;

                (2)0.5mlofpre-madenegativecontrolanti-miRNAlentiviruswithtiter at~1×107 IFU/ml;

 Note: pleaseprovidethemiRNA IDduringcheckoutinthe“Comments”field,or emailus themiRNAtargetIDoritsmaturemiRNAsequencethatyouwanttoinhibitorrepress.

Reference:

KluiverJ,GibcusJH,HettingaC,AdemaA,etal.(2012)RapidGenerationofMicroRNASpongesforMicroRNAInhibition.PLoSONE7(1):e29275.