Pre-madelentivirusexpress RenillaLuciferase reporterunderaminimalpromoterinsertedwith8tandemrepeatsofGliresponsiveelement,fordetectingHedgehog signalingpathwayacitvity.
Thislentivirus alsocontainthepuromycinselectionMarkerundertheconstitutiveRSVpromoterforantibioticselection. See ProductManual fordetails(.pdf).
Ifdesired,youcanalsousea pathway“ControlLentivirus”toestablishtheNo-responsecontrolprofiletoyourpathwaystimulus.Thecorresponding pathwaycontrollentivirusis:CAT#:Path-Ctr3.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS.
Cat#:LVP948-P