Pre-madelentiviralparticlesexpressingafusiontargetof(CFP-humanActingene). Thehuman betaactin(NCBIaccessionID:NM_001101)wasfusionedatitsN-terminalwithCFPprotein,expressedundera constitutive suCMVpromoter, containingapuromycin antibiotic Markerunderthe RSVpromoter.Itcanbeusedto visualizesub-cellularstructurewheretheactinwastargeted.
Actinsarehighlyconservedproteinsthatareinvolvedincellmotility,structure,andintegrity.Thisactinisamajorconstituentofthecontractileapparatusandoneofthetwononmusclecytoskeletalactins.
Pleaseseeproductmanualfordetails.
Amount:200ul/vial(1×10e7 IFU/ml), providedinDMEMmediumwith10%FBSand60ug/mlPolybrene(10x).
Cat#:LVP446-C