PremadenegativecontrolshRNAexpressionparticles underhumanU6promoter.NegativeshRNAcontrolscontainingainsertthatdesignedhasnohomogoustoanyhumanormousetranscripts(shouldnottargetanyknownhumanormousegenes).Thesecontrolsserveasausefulreferenceforinterpretationofknockdownresults.
ParticlescontainsaRFP-PuromycindualselectionMarker.RFPfluorescentsignalprovidea convenientmethodtoobservethevirustranductionundermicroscope,andthetransucedcellscanbesorted/selectedviaeitherflourescentsignalorPuromycin.
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)inDMEMmediumwith10%FBSwith10xpolybrene.
Cat#: U6(shRNA-Ctr)-RP.