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Gentarget/Luciferase2ACRE (Puro) lentiviral particles/LVP409/1 Ea

Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:fireflyluciferaseandnuclearpermeableCRE recombinase,underthesamesuCMVpromoter.APuromycinMarkerwasexpressedunderRSVpromoter,whichallowtoselectthepositivetransducedstablecellsforlongtermcultureviaantibioticselection.

BothlucifeaseandCREarehighlyactive,bestforbothenzymes'assaysInvivoorInvitro.CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.This nuclearpermeant CRE(NLS-CRE) increasestherecombinationeventsinvivo. PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

LuciferaeandNLS-CRE areconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired.Luciferasesignalprovidesaconvenient,realtimemonitoringthe particlesperformance. 

Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at 1x107IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP409


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