This line of embryonic mouse hippocampal cell lines is based on a proprietary platform technology which has enabled the creation of 4 phenotypcially distinct immortalized cell lines; immortalized from male and female Swiss Webster mouse Hippocampal primary cultures by retroviral transfer of SV40 T-Ag.

The Hippocampal cell lines are easy to culture, have efficient transfection rates and have robust gene and protein expression.As such, these cell lines enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, reproduction and metabolic disorders, amongst others.

Further details regarding the cells can be found in the following paper:

• Neuroscience (2010) by Gingerich et al.

Download the Hippocampal Cell Line Screen Profile

Immunofluorescence Analysis

Anti-human SNAP-25, Alexa Fluor488, (clone SP12), (CL2705AF4) staining on Embryonic Mouse Hippocampal Cell Line mHippoE-5 cells (CLU197) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).

Anti-human SNAP-25, Alexa Fluor647, (clone SP12), (CL2705AF6) staining on Embryonic Mouse Hippocampal Cell Line mHippoE-5 cells (CLU197) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).

Anti-human Syntaxin-1, Alexa Fluor488, (clone SP6), (CL2706AF4) staining on Embryonic Mouse Hippocampal Cell Line mHippoE-5 cells (CLU197) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).

Anti-human Munc18-1, Alexa Fluor488, (clone 1B9), (CL2710AF4) staining on Embryonic Mouse Hippocampal Cell Line mHippoE-5 cells (CLU197) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).