近年来，荧光蛋白成为在
显微镜下研究蛋白定位，动态变化的新型工具。但是，想要得到完整的图片，数据必须结合其他的荧光融合蛋白信息才能完整呈现。目前，缺乏高特异性，稳定，高效的试剂成为结合细胞生物学和生物化学研究方法的桎俈。Chromotek公司Nano-Traps系列产品为您带来真正高效，稳定的新一代荧光蛋白产品体验！
来自于alpaca羊驼的偶联有单价矩阵基质（
琼脂糖珠，
磁珠以及多孔板基质）的单结构域的
抗体片段。
产品描述：
与agarose beads结合partical size~80μm
存储
缓冲液：20% EtOH
储存方法：室温运输，收到后4℃保存一年。勿冻存。
产品特色：
1生化分析荧光融合蛋白强有力工具
2 孵育时间短（5-30min）
3 从细胞提取物或细胞器中定量分离融合蛋白及结合（暂时性）因子
4 超低的非特异性结合影响
5 无污染的普通抗体重轻链
产品应用：
1 Pull down实验
2 IP/Co-IP
3 质谱分析
4 酶活性测定
5 染色体免疫共沉淀
产品主要包括以下两个系列GFP-Trap和RFP-Trap荧光蛋白系列产品
GFP-Trap 绿色荧光蛋白系列产品
产品展示：
FP-Trap_A
gta-20 20 reactions（500 ul resin）
gta-100 100reactions（2.5 ml resin）
gta-200 200 reactions（5 ml resin）
gta-400 400 reactions（10 ml resin）
RFP-Trap 红色荧光蛋白系列产品
产品展示：
GFP-Booster；RFP-Booster
该产品出自ATTO公司优质的
荧光染料共价结合在小片段高特异性的GFP，RFP荧光蛋白上，可以达到重激活，增强，稳定荧光信号的作用。
产品展示：
FAQ
1、GFP-Trap®可识别的GFP衍生物类型有哪些？
(1)eGFP,wtGFP,GFP S65T
(2)TagGFP
(3)eYFP,YFP,Venus,Citrin
(4)CFP
不能识别：TurboGFP，所有的RFPs
2、RFP-Trap®可识别的RFP衍生物类型有哪些？
(1)mRFP
(2)mCherry
(3)mOrange
(4)mPlum
(5)mRuby
(6)mKate2
不能识别：DsRed、mRFPruby、TagRFP、所有GFPs
3、Nano-Trap®(GFP-Trap®/RFP-Trap®)的结合能力如何？
Nano-Trap®的结合能力取决于珠子。每10μL浆液，琼脂糖珠可结合2~3μg GFP，磁性颗粒可结合0.25-~0.5μg GFP。
4、可以从GFP-Trap洗脱结合蛋白么？
关于定量洗脱，可以将样品至于SDS
上样缓冲液中95℃煮10min（详见protocol），或者在0.2 M的甘氨酸（pH2.5）中孵化0.5~2min，随后用0.1体积的1M Tris碱中和。
5、是否可以从GFP-Trap洗脱出自然状态的结合蛋白，比如，在凝胶迁移实验或功能分析实验中？
可以尝试洗脱游离的GFP。但是，请注意，这种方法，不能定量洗脱出目标融合蛋白。
6、怎样才能避免非特异性的蛋白与GFP-Trap交互作用而结合？
关键操作步骤，是在孵化液中稀释洗涤剂的浓度。我们建议，洗涤剂为终浓度0.1%（如NP-40 或TX-100），且最终体积不少于0.4mL。另外，我们建议，要测定各种洗涤缓冲液的盐浓度，使其范围在150mM-500mM范围内，以去除非特异性结合的亲水性蛋白。
7、在binding过程中，N-端与C-端GFP融合蛋白之间是否存在差异？
GFP-Trap®对C-末端的GFP融合蛋白的亲和力稍高，若要消除这种差异，可以加长孵化时间（1-2h取代15-30min）
8、是否可以在组织样品（即变性缓冲液）中直接纯化GFP标记的融合蛋白？
原则上，GFP-Trap®即使在恶劣的缓冲条件下（如，RIPA缓冲液，含0.1%SDS或1M尿素），也非常稳定。
9、可结合的GFP-Trap®珠的GFP结构，是否有大小限制？
无。
10、如果在洗脱液中，有剩余的背景怎么办？
建议使用Chromotek的blocked particles（bab-20 or bmp-20）对样品进行预处理。
名家推荐：
Your GFP-Trap® is great! I have never seen such efficient reagent for pull down."
Prof. Dr. Tomoyuki Tanaka, Wellcome Trust Centre for Gene Regulation & Expression, University of Dundee
“We recently had excellent MS results with the GFP-Trap®.”
Dr. Gwyneth Ingram, IMPS, Edinburgh
“We have been pleased with the results from both of the products: GFP-Trap_A® and GFP-Trap_M®”
Katharine S. Ullman, Ph.D., Assoc. Professor, Huntsman Cancer Institute, University of Utah
“The F2H® approach is the missing link between biochemistry and cell
BIOLOGy”
Prof. Sir David Lane, p53 Lab, A*Star, Singapore
“I just wanted to give you a quick update on our experiences testing your GFP-Trap®. This will be quick because our experience is that they work fantastically well!”
Prof. Dr. A.I. Lamond, Wellcome Trust Center, University of Dundee
“We were gob smacked when we did our own IP and saw the glowing green GFP-Trap® beads...”
Prof. Dr. Laura Trinkle-Mulcahy, University of Ottawa
“The GFP-Trap® worked very nicely to pull-down our actin binding protein...”
Prof. Dr. Evelyne Friederich, University of Luxembourg
“I am brim over with enthusiasm for your GFP-Trap®; great product and great idea; Hats off!”
Dr. Monika Jedrusik-Bode, MPI for Biophysical Chemistry, Göttingen
"GFP-Trap® is the best beads ever!"
Dr. Kenkyo Matsuura, Department of Pharmacology and Cancer Biology, Duke University
“When I used antibody for immunoprecipitation, strong background was usually hard trouble. I think that GFP-Trap® is revolution.“
Takeshi Mizuno, Ph. D., Advanced Science Institute, RIKEN (Institute of Physical and Chemical Research)
"The results obtained with the GFP-Trap® are much more clean (…) I am thus very happy about the GFP-Trap_A® product!”
Florence Besse, PhD, Univ. Nice Sophia-Antipolis
“Man erlebt es in der Wissenschaft ja eher selten, dass ein neues Tool in den eigenen Händen genau so gut funktioniert, wie man es sich erhofft hatte - mit der GFP-Trap_A® war das bei mir der Fall! Das hat mich wirklich überzeugt!”
Friederike Althoff, Institut of Zoology, University of Zürich
“The beads GFP-Trap®_M are fantastic!”
Francesca Sicilia, Dipartimento di Biologia Vegetale, Universitá di Roma "La Sapienza"
"Your GFP-Trap® is such a powerful tool for bio-sensing applications! Stable, specific, sensitive, soluble...it has all the ideal features of a capturing molecule. And it comes at a very competitive price."
Dr. Eduardo Della Pia, Nano Science Center, University of Copenhagen
"...the beads were glowing purple so we knew there was a ton of mCherry on them! I think the RFP-Trap® is a really nice reagent..."
Prof. Dr. Laura Trinkle-Mulcahy, University of Ottawa
"We have found your trap tools extremely useful, the purification effect is way superior to most of our antibodies. This tool proved very helpful in our research and we are practically addicted on it."
Dr. Ondrej Plihal, Institute of Microbiology, Department of Ecology
"I tested the RFP-Booster for labeling Drosophila oocytes using the suggested protocol. The Booster was specific and had double the fluorescence intensity as the fixed mCherry control. Even more exciting was that the dye was wonderfully stable. We imaged 9000 frames and had very little (if any) bleaching."
Dr. Timothy Weil, Oxford
"I am a big fan of all GFP/RFP-Trap® for Co-IP experiments and we use it regularly. Same thing for the labeled antigen specific nano-bodies."
Dr. Harald Wodrich, University Bordeaux
"The 5F8 antibody is great. We have beautiful staining in the olfactory bulb for our neurons electroporated with RFP-encoding vector"
Manav Pathania, School of Medicine, Yale University
