下一代固相染色质免疫沉淀（ChIP）分析技术 — Chromatrap®染色质免疫沉淀（ChIP）系列

Chromatrap®使用PorvairSciences专利过滤器，与英国斯旺西大学的生殖生物学和妇科肿瘤组合作开发出更灵敏的染色质免疫沉淀技术。特别适合于微量或低丰度生物样品。
Chromatrap®ChIP采用与蛋白质A或G结合的固相多孔聚合物来捕获染色质、Chromatrap®试剂盒（专利号：GB2482209）采用与蛋白质A结合的固相多孔聚合物来捕获染色质，较磁珠法的效率得以极大提高。过去的琼脂糖珠或磁珠法工艺复杂，涉及分离、移液和再悬浮等操作，相比之下，Chromatrap离心柱法的优势显得尤为突出。
独立实验表明，Chromatrap法比传统珠式工艺的DNAPullDown水平高出25倍，DNA富集的信噪比尤为显著，要比常见的工艺高出2至3倍，即使沉淀反应的用品用量过少至50ng或过多至3000ng，都能得出十分稳定的结果。
Chromatrap®ChIP简化qPCR分析流程5小时内制备染色质
 

EpigeneticsResearchersEnthuseOverChromatrap®ChIPtechnology 
1.BrianJ.Engel,aBiochemistry&CellBIOLOGyresearcheratRiceUniversity,USAcommented"WhatIlikemostabouttheChromatrapkitcomparedtotrADItionalbead-basedChIPassaysistheeaseofuse,speedandreliABIlity.Thereisnoworryaboutaccidentallylosingbeadsduringwashesandreducingoutputchromatinwhenusingthecolumn-basedmethod".Hiscolleague-CurtWarrenadded"WehavefoundthattheChromatrapkitisquick,reliableandflexIBLe. SavingreagentsandtimewiththiskitmakesiteasierandmorefeasibletoconductmorethoroughandeffectiveIPbasedexperiments. WiththeenhancedreproducibilityusingChromatrap,Iwouldnotgobacktobead-basedChIPandtheassayhasbeenfullyendorsedandadoptedwithinourresearchteam".
 
2.ProfessorSteveConlan,HeadofMolecular&CellBiologyatSwanseaUniversity,UKcommented"Chromatrapstreamlinesthecomplicatedexperimentalprocedureassociatedwithchromatin-IPmakingitamuchquickeranduser-friendlyassaycomparedtoconventionalbeadbasedassays.Usingthiskitweobtainmuchmoreconsistentdata,andmostimportantlycanusemuchlessinputsample.WehavemadeapermanentswitchtousingChromatrapforallourcelllineandprimarycellbasedassays,andfullyendorsethetechnology".Researchassistant-DrHelenWhitelandadded"WhatIlikeabouttheChromatrapassayistheeaseofuseandquickmethodallowingmetoanalysemydatainthesameday".