1.哈佛大学医学院,微生物学和免疫生物学系
J.Virol.,Jan2012;86:1145-1157.ThomasS.Postleretal.
AntibodyreactedwithrecombinantSIVmac239proteinsgp140andgp120(
ImmuneTechnologyCorp.,NewYork,NY)
2.哈佛大学医学院,新英格兰灵长类动物研究中心
J.Virol.,Jan2011;85:582-595.ElizabethStanselletal.
CellsandpurifiedusingtheHistagwerepurchasedfrom
ImmuneTechnologyCorp.,NewYork,NY:SIVmac239,SIVsmE660,HIV-2Ben,HIV-2.ForSIVandHIVproteinspurchasedfrom
ImmuneTechnologyCorp.
3.哈佛大学医学院,新英格兰灵长类动物研究中心
J.Virol.,Sep2010;84:8998-9009.ElizabethStanselletal.
HIV-1JRCSFgp120,HIV-1p1006_11.C3.1601gp120,HIV-1consensusBgp120,HIV-1consensusA2gp120,HIV-1consensusCgp120,SIVcpzEK505gp120,andSIVcpzMB66gp120producedfromHEK293cellswerepurchasedfrom
ImmuneTechnologyCorp.,NewYork,NY.GNA
ELISA.ForthecaseofSIVandHIVproteinspurchasedfrom
ImmuneTechnologyCorp.
4.哈佛大学儿童医院,儿科系
CellstructureVolume18,Issue12,8December2010,Pages1632–1641.MichaelM.Freemanetal.
gp120derivedfromtheisolateDu156.12from
ImmuneTechnologyCorp.(NewYork,NY).Gp120core,soluble2Dand4DhumanCD4wereexpressedininsectcells.
5.美国国立卫生研究院,NIAID疫苗研究中心
J.Virol.,Aug2012;86:7760-7770.LukasFlatzetal.
TheELISAplatewascoatedwitheitherSIVmac239gp140orHIVgp120B(
ImmuneTechnology,NY).
6.美国国立卫生研究院,约翰·霍普金斯大学,美国食品和药物管理局(FDA)
VaccineVolume28,Issue44,18October2010,Pages7193–7201.ChiaChuetal.
Wisconsin/67/2005X-161,Cat.No.IT-003-004WI,
ImmuneTechnologyCorp. 7.美国国立卫生研究院,NIAID疫苗研究中心
J.Virol.,Jul2008;82:6200-6208.Chih-JenWeietal.
rabbitpolyclonalanti-HAantibody(
ImmuneTechnology,NewYork,NY).
8.FDA,美国食品和药物管理局病毒产品事业部
J.Virol.JVI.01596-12;5September2012.SwatiVermaetal.
TheH5N1-rHA1ormammaliancellderivedglycosylatedHA0proteins235Page12.12(
ImmuneTechnology Corp)werecoupledtoaGLCsensorchipwithaminecouplingin236thetestflowcells.
9.FDA,美国食品和药物管理局病毒产品事业部
PLoSONEFebruary2011,Volume6,Issue2,e17297.HanxinLuetal.
PurifiedmammaliancellderivedH5N1HA1ortheHA0proteinsobtainedfrom
ImmuneTechnologyCorp.
10.FDA,美国食品和药物管理局病毒产品事业部
PLoSONEJuly2010|Volume5|Issue7|e11548.SurenderKhuranaetal.
AmammaliancellderivedrecombinantfulllengthHA(HA0)(from
ImmuneTechnologyCorp,NY)weretestedforbindingtofetuincoatedonbiosensorchips.
11.德国Georg-Speyer-Haus生物医学研究所
J.Virol.,Oct2010;84:10131-10138.XavierDervillezetal.
Overnightwith100ngstrain89.6gp120(
ImmuneTechnology)perwell.Aftertheplateswere...experimentswiththegp120variants(
ImmuneTechnology),purifiedectodomainofhumanCD4(
ImmuneTechnology).
12.华盛顿FredHutchinson癌症研究中心。
J.Virol.,Sep2012;86:9566-9582.LeslieGooetal.
PurifiedrecombinantsubtypeC(BL035.W6M.ENV.C1;
ImmuneTechnology)proteinswereusedasgp120.
13.加利福尼亚州MedImmune公司
J.Virol.,Jul2010;84:6570-6577.WeijiaWangetal.
Rabbitanti-HA1(H5N1)antiserumwasobtainedfrom
ImmuneTechnologyCorp.(NewYork,NY).Generationofrecombinantviruses...beforeincubationwithrabbitanti-HA1(H5N1)antiserum(
ImmuneTechnologyCorp.,NewYork,NY),followedbyincubationwithhorser
ADIsh.
14.杜克大学医学院人类疫苗研究所
J.Virol.,Jul2011;85:7029-7036.GuidoFerrarietal.
Recombinantgp120HIV-1proteinfromtheWITO(gp120WITO)early-infectionEnvclone(GenBankaccessionnumberAY835451)
(ImmuneTechnologyCorp,NewYork).
15.芝加哥伊利诺伊大学
J.Biol.Chem.,Jul2011;286:23975-23981.JessicaCeligoyetal.
Aseriesofmonoclonalantibodies.gp120fromstrainYU2wasobtainedfromthe
ImmuneTechnologyCorp.gp120fromstrain96ZM65wasobtainedfromtheNationalInstitutesofHealthAIDSResearch.
16.纽约罗切斯特大学医学中心
J.Immunol.,May2011;186:5514-5521.F.Eun-HyungLeeetal.
PurifiedH1:2009California(3mug/ml;
ImmuneTechnology,NewYork,NY).
17.休斯敦贝勒医学院
J.Virol.2011,85(20):10639.LisaGolebiewskietal.
PrimaryantibodiesusedweretoNS1(
ImmuneTechCorp.).
18.斯坦福大学化学工程系
BiotechnologyandBioengineering,16AUG2012.JohnP.Welshetal.
CommercialHAconsistedofaminoacids18–529ofthe2009pandemicH1N1strain(HA(ΔTM)(A/California/07/2009(H1N1)))followedbyaHis6purificationtagattheC-terminus(
ImmuneTechnologyCorp.,NewYork,NY).
19.美国VaxInnate公司,默克公司
Vaccine,18September2012.GeLiuetal.
HA0AN,recombinantHA(aminoacids18–530)ofA/Anhui/1/2005expressedfrommammaliancells(
ImmunetechnologyCorp.,NewYork,NewYork).
20.密歇根州立大学
BiosensorsandBioelectronics,15December2010,Pages1346–1352.TracyL.Kamikawaetal.
Hemagglutinin(HA)protein(VN04-2),A/Vietnam/1203/04(H5N1)(ascites,Mouse),NR-2728.Thefollowingwerepurchasedfrom
ImmuneTechnologyCorp.(NewYork,NY):(1)6xHistaggedH5hemagglutinin(HA)proteinfrom293cell.
21.加州大学药学院
Vaccine,23July2009,Pages4672–4683.DouglasS.Watsonetal.
Recombinantgp140ELISAswereperformedasfollows:Ba-1gp140(
ImmuneTechnologyCorp,NewYork,NY).
22.威斯康辛大学
PLoSPathog2011,7(11).JasonT.Weinfurteretal.
PurifiedHAproteinsexpressedin293cellswerepurchasedfrom
ImmuneTechnologyCorp.(NewYork,NY)foruseinELISAs.
23.纽约大学医学院
VirologyVolume427,Issue2,5June2012,Pages198–207.MiroslawK.Gornyetal.
RecombinantHIV-1proteins.Nineteenrecombinantgp120swerepurchasedfrom
ImmuneTechnologyCorp.
24.澳大利亚洛伊癌症研究中心
J.Biol.Chem.,Dec2010;285:40072-40080.ImanAzimietal.
anti-gp120/160rabbitpolyclonalantibody(ImmuneTechnology).
25.意大利bologna大学
GlycoconjugateJournal Volume27,Number5(2010),501-513.AnnaKabanovaetal.
IT-001-0029p),andgp120JRFL(aa34–518,GenBankNo.U63632,catalogno.IT-001-0024p)werepurchasedfrom
ImmuneTechnologyCorp.(NewYork,US).PolymunandImmuneTechnologyrecombinantproteinsareexpressedinCHOand293Tcells.
26.法国Biceˆtre医院内科和传染病系
AIDS:15May2009-Volume23-Issue8-p897-906.Lambotte,Olivieretal.
CEM.NKRcellswereusedastargets,coatedwithrecombinantgp120representingthesequencesoftheMN,JRFL,andQH0692isolatesrgp120JRFLandrgp120QH0692(
ImmuneTechnologyCorp,NewYork,USA).
27.瑞士Linköping大学
KarlhansFruCheetal.
HIV-1BaLgp120proteinwaspurchasedfrom
ImmuneTechnologyCorp.,NewYork,NY.
28.加拿大Medicago公司
PlantBiotechnologyJournal,pages930–940,December2008.Marc-AndréD’Aoustetal.
Anti-H5immunoblottingwasperformedbyincubationwithrabbitanti-H5(A/Vietnam/1203/2004)antibodies(
ImmuneTechnology,NewYork,NY,USA;Cat#IT-003-005V).
29.香港大学新发传染性疾病国家重点实验室
PNAS,Jun2008;105:8091-8096.Bo-JianZhengetal.
InfluenzaAviralproteinsNPfromH5N1strainA/Indonesia/5/2005,HA1fromH5N1strainA/Vietnam/1203/2004
(ImmuneTechnology). 30.香港大学生化系
BMCImmunology2012,13:54.SongyueZhengetal.
H5antigen(H5N1,A/Vietnam/1203/2004,
ImmuneTechnologyCorp.)in100μlofPBScontaining0.2%BSA.
31.北京理工大学生科院,中科院武汉病毒所
Anal.Chem.,2012,84(19),pp8364–8370.JianHaoetal.
anti-H3protein(vacciniavirus)mousemonoclonalantibody(mAb)from
ImmuneTechnologyCorp(USA).