Jellagen/Research Grade Jellyfish Collagen/1141Q16

Jellagen® purified next generation jellyfish collagen for cell culture, tissue engineering and regenerative medicine

Jellagen are pioneering the manufacture and development of this next generation, self coating collagen for cell culture and medical applications. As jellyfish collagen is not derived from mammals or fish, it has the advantage of being disease vector and BSE free.

Jellyfish collagen most resembles Type I “like” collagen but according to literature has been referenced as having collagen Type II-“like” and Type V “like” properties.

This product can be purchased as either a 0.3% and 0.6% (3 mg/ml and 6mg/ml) solution of jellyfish collagen in 0.1M acetic acid. It is intended to produce (dried) thin layer coatings on tissue culture plates/flasks to facilitate attachment of anchorage dependent cells.

How to use

This self coating collagen solution can be used for all existing collagen protocols and applications. It has also been tested in 2D culture with mammalian and human primary cells to verify it is applicable for routine laboratory cell culture research. Jellyfish collagen offers Type I “Like” and Type II “Like” scaffold properties.

• Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
• Type II collagen is the predominant fibrous component making up 90–95% of the primary collagen and 40–70% of the total dry weight of articular cartilage.

Jellyfish collagen has been shown in cell culture as an attachment substratum. Examples of human primary and stem cells include, Mesenchymal Stem Cells, Fibroblasts, Hepatocytes, Endothelial cells, Keratinocytes, Chondrogenic Progenitor Cells, Urine Derived Stem Cells, Cardiomyocytes have all been cultured successfully on films or gels of jellyfish collagen. Jellyfish collagen differs from mammalian collagens by their lower hydroxyproline composition which may affect thermal stability. This can be overcome by the use of standard chemical cross-linking protocols.