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Nichirei/Principle and Protocol/414132F/414132F

Principle and Protocol

-For Human Tissue Sections

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.
Feature
-Histofine®Simple Stain MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab" fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with human serum.
Principle & Procedure
Advantages
1.Simplified staining steps (more).
2.High sensitivity (more).
3.Unaffected by endogenous biotin in the tissue (more).
4.Ready to use.
Staining Result
-Mouse anti-Progesteron Receptor antibody (clone:1A6)
Human breast cancer (treated with high temperature epitope unmasking method) stained with -Histofine®Simple Stain MAX PO(M) and DAB chromogen.Note nuclear staining of breast cancer cells.
-Rabbit anti-S-100 protein antibody
Human colon stained with -Histofine®Simple Stain MAX PO(R) and DAB chromogen.Note cytoplasmic staining of nerve cells.
-Mouse anti-Estrogen Receptor antibody (clone: 1D5)
Human breast cancer (treated with high temperature epitope unmasking method) stained with -Histofine®Simple Stain MAX PO (MULTI) and DAB chromogen.Note nuclear staining of breast cancer cells.
ProductDetection systems.
Product NameSlidesVolumeCodeFor use withMark in EU
-Histofine®Simple StainTM MAX PO (MULTI)1701x17ml414151FMouse and rabbitprimary antibodiesCE
5003x17ml414152F
15009x17ml414154F
-Histofine®Simple StainTM MAX PO (M)1701x17ml414131FMouseprimary antibody
5003x17ml414132F
15009x17ml414134F
-Histofine®Simple StainTM MAX PO (R)1701x17ml414141FRabbitprimary antibody
5003x17ml414142F
15009x17ml414144F
-Histofine®Simple StainTM MAX PO (G)1701x17ml414161FGoatprimary antibody
5003x17ml414162F
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