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OXFORD EXPRESSION TECHNOLOGIES PRODUCT OVERVIEW188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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OXFORD EXPRESSION TECHNOLOGIES PRODUCT OVERVIEW

BaculovirusExpressionProducts
FromTheExperts
 

Baculovirusexpressionvectors
Thesimplestandthequickestbaculovirusproteinexpressionvectors.Geneticallyoptimizedforproducing100%recombinantvirusinasinglestepbyeliminatingtheneedforplaquepurification.
 TheoriginalflashBACvectorisstillagreatchoiceformostprojectswherethegenetobeexpressedislikelytobesimpleandexpressedinthecytoplasmornucleus.
 flashBACGOLDisenhancedbythedeletionofthechitinaseandcathepsinproteasegenes.Itprovidessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.
 ThelatestdevelopmentoftheflashBACtechnology,flashBACULTRA,containsdeletionsofthechitinaseandcathepsingenes,asabove,butalsodeletionsofthep10,p74andp26genesandprovidesthebestchoiceforthemostdifficulttoexpressproteins.Suitableforcytoplasmic,nucleus,secretedandmembraneproteins.
 AcombinationofflashBAC,flashBACGOLDandflashBACULTRAinasinglekit.3reactionsofeachallowsyoutoselectthebestexpressionvectorforyourprotein.
Baculovirusproteinexpressionkits
TheperfectchoiceforscientistswantingtoupgradetotheflashBACbaculovirusexpressionplatform.AnALL-IN-ONEkitcontainsallthecomponentsneededtogetstarted with recombinant protein expression in insect cells. Kit contains flashBAC ULTRAbaculovirus,baculoFECTINtransfectionreagent,controltransferplasmid,baculoGROWinsectcellmediaandSf9insectcells.Thecomponentsinthekithavebeenoptimisedtocomplementeachotherandtoproducebesttitresofrecombinantvirusandyieldsofproteinstohelpensureyoursuccess.
Baculovirustitration
 AQ-PCRbasedone-stepvirustitrationkitwhichcangiveanaccurateresultwithin2hourswithouttheneedforplaqueassaysorimmunoassays.
IsanovelALL-IN-ONEvirusextractionandtitrationkit.BothbaculovirusDNAextractionandQ-PCRbasedquantificationhavebeencombinedintooneconvenientkit.VirusDNAextractionrequiresnospincolumnsandiscarriedoutinasinglestepusingathermocycler.Theprimers,probe,mastermixandcontrolsforQ-PCRareallincludedinthekit.
Baculovirusexpressionandtitration
 TheconvenienceandqualityofbothbaculoQUANTandanyvariantoftheflashBACrangecombinedintoonegreatvaluekit!.
Transferplasmids
AselectionoftransferplasmidsthatallowsimplecloningandfacilitatehomologousrecombinationintotheflashBACsystem.Thesecanofferfurtherimprovementstoproteinexpressionandmodification.
 DesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedrin(polh)promoter.ContainaColE1originofreplicationandanAmpicillinresistancegeneforselectioninE.coli.
Thesevectorsgreatlyeasethepurificationoftherecombinantproteinssincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose.ThevectorsareavailablewithachoiceofeitherNorCterminalHistags.
 ProteinexpressionisdrivenbytheAcMNPVp6.9promoterwhichprovidesearlierexpressionthanthepolhpromoterusedinpOET1andpOET2.Suitablewhenexpressingproteinsthatrequireextensiveposttranslationalmodifications.
IsadualpromoterbaculovirustransfervectordesignedforhighlevelexpressionoftwoforeigngenessimultaneouslyunderthepowerfulAcMNPVpolhpromoterandtheverylatep10promoter.Thisvectorisalsosuitablefortheexpressionofmulti-subunitproteins.
TransfectionReagents
 Thisnovelnanoparticlebasedtransfectionreagenthasbeenespeciallydevelopedandoptimisedforthetransfectionofinsectcells.Withitsuniquecharacteristicsityieldsgreaterlevelsoftransfection.
Insectcells
 TheSf9cellsareadaptedtoserum-freesuspensioncultureinbaculoGROWmedia.Thecellscanbeusedfortransientorstableexpressionofrecombinantproteins,asamonolayerfortransfectionandproductionofrecombinantbaculovirusorforthepropagationofbaculovirusstocks.
AreSf9cellsthathavebeenengineeredtostablyexpressanadditionalprotein.ThepresenceofthisproteinleadstoprolongedlongevityandincreasedrecombinantproteinproductioninbaculovirusinfectedSuperSf9cellscomparedtostandardSf9cells.Thereare3SuperSf9celllines,eachcapableofenhancingtheexpressionofdifferenttypesofrecombinantproteins.
Insectcellmedia
 Isaserum-andprotein-freemedium.ThemediumhasbeenspeciallyformulatedtofacilitatethegrowthofSpodopterafrugiperdacelllines(suchasSf9andSf21),DrosophilaS2cellsandothercellssuchasTrichoplusiani(T.niorHi-5)cells.baculoGROWcanbeusedforculturingadherentcellsandsuspensioncultures.
Transferplasmidsequencingprimers
 
 ThesequencingprimersareusedtosequenceinsertsclonedintothemultiplecloningsiteofanyofthepOETtransfervectors

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