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Oxford Expression /pOET4 Transfer Plasmid/200105/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Oxford Expression /pOET4 Transfer Plasmid/200105/1 Ea

Description:

pOET4(cat#200105)(10ug)isabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthelateAcMNPVbasic(p6.9)promoter. Usingthispromoterwillprovideearlierexpressioncomparedtothepolyhedrinpromoter. Thishasbeenshowntobebeneficialwhenexpressingproteinswhichrequireextensivepost-translationalmodificationsi.e.glycosylation.Thevectorissmallerthanotheravailabletransfervectors(4536bp)whichgreatlyfacilitatethecloningsteps.IthasabacterialoriginofreplicationandanampicillinresistancegeneforselectioninE.coli.TheAcMNPVsequencesflankingthegeneinthetransfervector’sMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolyhedrinlocus.Thepolyhedrinsequenceshavebeenreplacedbyamultiplecloningsitecontaininguniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation.pOET4iscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.

Variations

pOET3 hasthesameMCSaspOET4butinthereverseorientation.

 

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