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BacPAK6Sec+linearizedbaculovirusDNAisthelatestbaculovirusexpressionsystemtocomeoutoftheOETlabs.BytakingtheoriginalBacPAK6systemandgeneticallymodifyingit,BacPAK6Sec+isabletoincreasetheyieldofrecombinantproteinandtheeaseofharvesting.
BacPAK6Sec+containsadeletionofthechitinasegene(chiA)fromtheoriginalBacPAK6viralbackbone.
Chitinasehasbeenshownbybothconfocalandelectronmicroscopytolocatetotheendoplasmicreticulum,whereitisdenselypackedinaparacrystallinearray,whichseverelyinhibitsthefunctionandefficacyofthesecretorypathway.BydeletingthechiA,secretionoftargetproteinsintothecytoplasmisincreased,greatlyenhancingtheyieldofharvestablerecombinantprotein.
TheconceptofdeletingthechiAgenecameafterthesuccessoftheflashBAC™platformthatutilizesthesamegeneticdeletionforincreasedrecombinantproteinyield.TofindoutmoreabouttheflashBAC™system,clickhere.
BacPAK6linearDNAistheoriginalconvenient,highlyefficientreagentforgeneratingrecombinantbaculovirusexpressionvectors. Itcomprisesamodified Autographacalifornica nucleopolyhedrovirus(AcMNPV)genomewith lacZ insertedinplaceofthenativepolyhedringenecodingregionforconvenientblue/whiteselection.
DigestionofBacPAK6DNAatthreesiteswithBsu36Iremoves lacZ andpartofORF1629,whichisanessentialAcMNPVgene. ThelinearvirusDNAisunabletoinitiateareplicationcycleininsectcells.
Co-transfectionofinsectcellswithlinearBacPAK6DNAandacompatIBLetransfervectorcontainingthecompleteORF1629andaforeigngeneofinterestresultsinrecombinationandrestorationofthecircular,infectiousvirusgenome. Over95%ofthevirusderivedfromtheco-transfectioncomprisesrecombinantvirus. Usually,asingleplaqueassaytitrationincubatedwithX-galandcounterstainedwithneutralredissufficienttodifferentiateparentalbluefromrecombinantwhite(colourless)plaques. ThesecanbereADIlyisolatedandamplifiedtoworkingstocksofpurifiedrecombinantvirusinafewdays.
TheBacPAK6Sec+reagentfromOETLtdcomprisestransfection-ready,linearized(Bsu36I-digested)virusDNA. Itisavailablein5,24and96reactionpacks. Forbulkpurchases,pleasecontactusoninfo@oetltd.com.
WeofferapromotionaldealonallBacPAK6productstoincludeafree3reactionflashBAC™kittohelpyoudecideifmakingtheswitchistherightchoiceforyourlab.
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g