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flashBAC™istheeasiestandthequickestbaculovirusproteinexpressionsystemavailable.TheflashBAC™expressionvectorsystemisanewplatformtechnologyfortheproductionofrecombinantbaculoviruses,geneticallyoptimizedforproducing100%recombinantvirusinasinglestepthuseliminatingtheneedforplaquepurificationorotherscreeningsystems.Thegeneticoptimizationnotonlypreventparentalvirusfromreplicating,butalsosignificantlyincreasesproteinyield.TheflashBAC™expressionsystemhasbeenspecificallydevelopedforoptimalproteinproductionininsectcells.TheflashBAC™DNAisabaculovirusgenomethathasbeengeneticallyoptimisedtofunctionasarecombinantproteinexpressionvector.Thesystemoffershighlevelsofproteinexpression,iseasytouseandisalsobackcompatIBLewithalltransfervectorsbasedonhomologousrecombination.InthiswaytheflashBAC™systemhasbecometheexpressionofchoiceforthoseneedingoptimalproteinexpressionininsectcells,andincreasinglyinmammaliancellsviatheBacMamsystem.
ThereareaseriesofflashBAC™expressionvectors,eachvaryinginthenumberofgenesdeletedfromthebaculovirusgenometosuitexpressionofdifferentproteins. TheseSelectionBoxesenableyoutotryoutdifferentversionsofflashBAC™inoneconvenientkit:SelectionBoxescontaineitherasingle3reactionkitof flashBAC™,flashBACGOLDandflashBACULTRA(Box1)orasingle3reactionkitof flashBAC,flashBACGOLD,flashBACULTRAandflashBACPRIME(Box2).TheoriginalflashBACexpressionvectorisagoodchoiceforprojectswherethegenetobeexpressedislikelytobesimpleandexpressedinthecytoplasmornucleus.flashBACGOLDisenhancedbythedeletionofthechitinaseandcathepsinproteasegenes.Itprovidessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.flashBACULTRAcontainsdeletionsofthechitinaseandcathepsingenes,asabove,butalsodeletionsofthep10,p74andp26genesandprovidesthebestchoiceforthemostdifficulttoexpressproteins;uitableforcytoplasmic,nucleus,secretedandmembraneproteins.flashBACPRIMEcontainsnogenedeletionsandhasprovenusefulforthereleaseandsubsequentpurificationofintracellularVLPsandproteincomplexes. Ifitisastarterkityouareafter,baculoCOMPLETEistheperfectchoice.Thiskitcontainsallthecomponentsneededtogetstartedwithrecombinantproteinexpressionininsectcells.
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g