产品资料

Polyplus Transfection/jetPEI^®/Polyplus Transfection-163150/1 Ea

Well-suitedforadherentandsUSPensioncells
FastandefficientmethodstotransfectcellsforHTS
CompatIBLewithserumandantibiotics
Exceptionallyreproducibleresults
Reverse,batch&forwardprotocolsavailable

Specifications:

Reagent	jetPEI^®
Moleculedelivered	DNA
Applications	Plasmidtransfection HighThroughputScreening(HTS)
Celltypes	Adherentandsuspensioncells
Numberoftransfections	1mlofjetPEI^®issufficienttoperformupto2000transfectionsin96-wellplates.
Storage	4°C,stablefor12months(101-01N)toatleasttwoyears(otherpackagingsizes)whenstoredappropriately
Providedwith	101-01N,101-10N,101-40Nand101B-010Nareprovidedwith150mMNaClsolutionforcomplexformation

Summary:

jetPEI^{^®} transfectionreagentisalinearpolyethyleniminederivative,freeofcomponentsofanimalorigin,providinghighlyeffectiveandreproduciblegenedeliverytoadherentandsuspensioncells.
jetPEI^{^®} transfectionreagentisthereforeparticularlywellsuitedforautomatedormanualHTS(HighThroughputScreening)withthreeprotocolsavailable:reverse,batchandforward.

Orderinginformation:

|||101-01N##0.1ml##5ml##|||101-10N##1ml##50ml##|||101-40N##4x1ml##4x50ml##|||101B-010N##10ml##2x250ml##|||

Description:

3protocolstosuityourapplication

Inthe forwardprotocol,thecellsaresplitthedaybeforetransfectionandthejetPEI^®/DNAcomplexesareaddedtotheadherentorsuspensioncells.

The reverseprotocol isthemostappropriatewhentransfectingapoolofgenes,suchasaDNAlibrary(Fig.1).Inthisprotocol,thejetPEI^®/DNAcomplexesarepreparedordepositedinthewellspriortoadditionofthecells.Complexesarestableforupto4hours(Fig.2).

The batchprotocol hasbeendevelopedtoprepareahomogeneouspooloftransfectedcells.Forthispurpose,thecellsaretransfectedjustaftertrypsinization,whilestillinsuspension.Thisprotocolispreferredfordrugscreeningapplicationsandallowsrapidprocessing,onedayfasterthantheforwardprotocol.

jetPEI HTS Protocol — **Fig.1:jetPEI^®reversetransfectionprotocolforHTSapplication.**

Robusttransfectioncomplexes

Complexesformedwiththewater-solublepolymerjetPEI^{^®} andDNAallowefficienttransfectionforupto4hours,incontrasttolipid-basedreagentsandcalciumphosphate.Thustheyallowplentyoftimetodispensethecomplexesintotheplates(Fig.2).

jetPEI - Transfection Complex stability — **Fig.2:EffectofcomplexformationincubationtimeontransfectionefficiencywithjetPEI^®.**HEK-293cellsweretransfectedin96-wellplateswithpCMVLucandjetPEI^®followingthereversetransfectionprotocol.Luciferaseactivitywasmeasuredafter24h.

Batchtobatchreproducibility

HTSDNAtransfectionusingjetPEI^{^®} giveshighlyconsistenttransfectionefficiencyfrombatch-to-batch(Fig.3).

Efficientinawiderangeofcelltypes

jetPEI^® successfullydeliversgenestovariousadherentandnon-adherentcelllines,aswellasprimarycells(Table1).Over550publicationsusingjetPEI^® canbefoundinthePolyplus-transfectionDatabase.Inaddition,ouronlineDatabasegivesspecifictransfectionconditionsforover400celllinesandprimarycells.

jetPEI - Examples of successfully transfected cells lines — **Table1:SomecommoncelllinesandprimarycellssuccessfullytransfectedusingjetPEI^®.**

Superiortransfectionresults

jetPEI^® wascomparedtoseveralotherpopulartransfectionreagents(Fig.4).jetPEI^® wasfoundtoofferthebestperformance:highefficiencyandlowvariABIlity(smallstandarddeviation).

jetPEI vs competitors — **Fig.4:Transfectionefficiencyofaseriesofcommercialreagents.**HeLacellsweretransfectedin24-wellplatesinthepresenceof10%serum,using1µgpCMV-luciferaseaccordingtothemanufacturers’protocols.Luciferaseexpressionwasmeasured24haftertransfection.

Applications:

OurjetPEI^®reagentisperfectlywellsuitedforplasmidDNAtransfection,especiallyforHigh-TroughputScreening(HTS)application.

Readmore…

Citations:

HereisaselectionofrelevantreferencesusingjetPEI®,moreareavailableinourPolyplus-transfectionDatabase.Elkan-Miller,T.,Ulitsky,I.,Hertzano,R.,Rudnicki,A.,Dror,A.A.,Lenz,D.R.,Elkon,R.,Irmler,M.,Beckers,J.,Shamir,R.,Avraham,K.B.(2011). IntegrationofTranscriptomics,Proteomics,andMicroRNAAnalysesRevealsNovelMicroRNARegulationofTargetsintheMammalianInnerEar.,PLoSOne 6,e1819.Gerlo,S.,Haegeman,G.,VandenBerghe,W.(2008). TranscriptionalregulationofautocrineIL-6expressioninmultiplemyelomacells.,CellSignal 20,1489.Melkman-Zehavi,T.,Oren,R.,Kredo-Russo,S.,Shapira,T.,Mandelbaum,A.D.,Rivkin,N.,Nir,T.,Lennox,K.A.,Behlke,M.A.,Dor,Y.,Hornstein,E.(2011). miRNAscontrolinsulincontentinpancreaticbeta-cellsviadownregulationoftranscriptionalrepressors.,EMBOJ 30,835-4.Rogowski,K.,vanDijk,J.,Magiera,M.M.,Bosc,C.,Deloulme,J.C.,Bosson,A.,Peris,L.,Gold,N.D.,Lacroix,B.,Grau,M.B.,Bec,N.,Larroque,C.,Desagher,S.,Holzer,M.,Andrieux,A.,Moutin,M.J.,Janke,C.(2010). Afamilyofprotein-deglutamylatingenzymesassociatedwithneurodegeneration.,Cell 143,564-7.Zhao,L.J.,Kuppuswamy,M.,Vijayalingam,S.,Chinnadurai,G.(2009). InteractionofZEBandhistonedeacetylasewiththePLDLS-bindingcleftregionofmonomericC-terminalbindingprotein2.,BMCMolBiol 10,89.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Polyplus Transfection/jetPEI®/Polyplus Transfection-163150/1 Ea