产品资料

Polyplus Transfection/jetPEI^®-Macrophage/Polyplus Transfection-163151/1 Ea

Efficienttransfectionofprimarymacrophages,glial&dendriticcells
Easy-to-useprotocol
CompatIBLewithserumandantibiotics

Specifications:

Reagent	jetPEI^®-Macrophage
Moleculedelivered	DNA
Applications	DNAtransfectionofprimarymacrophages,glial&dendriticcells
Celltypes	Primarymacrophages,glial&dendriticcells
Numberoftransfections	0.5mlofjetPEI^®-Macrophagetransfectionreagentissufficienttoperformupto500transfectionsin24-wellplatesor160transfectionsin60-mmdishes
Storage	4°C,forupto12months
Providedwith	150mMNaCl

Summary:

jetPEI^®-Macrophagetransfectsprimarymacrophages,glial&dendriticcellsthatareconsidereddifficulttotransfect.Itcontainsamannose-conjugatedlinearpolyethyleniminethatbindstocellsexpressingmannose-specificmembranereceptors,suchasmacrophages.

Orderinginformation:

|||103-05N##0.5ml##50ml##|||

Description:

Transfectionofprimaryhumanmacrophages

jetPEI^®-Macrophageallowssuccessfultransfectionofmacrophagesderivedfrommonocytesmaturatedfor7daysinpresenceofGM-CSF(Fig.1).

jet-PEI-Macrophage-fig1-vc — **Fig.1:TransfectionofhumanmacrophageswithjetPEI^®-Macrophage.**HumanmacrophagesexpressingBeta-GalactosidaseaftertransfectionusingjetPEI^®-Macrophage.

HighertransfectionefficiencycomparedtoversatilejetPEI^®

PrimaryhumanmacrophagesandmurineRAW264.7cellsexpresssignificantlyhigherproteinlevelswhentransfectedwithjetPEI^®-Macrophage(Fig.2).

**Fig.2:ComparativeefficiencyofjetPEI^®-MacrophageversusjetPEI^®onprimaryhumanmacrophagesandmurineRAW264.7cellsin24-wellplates.**Primaryhumanmacrophagesweretransfectedinthepresenceof100U/mlGM-CSFand10%serum,using1µgpCMVLucand2µltransfectionreagent.MurineRAW264.7cellsweretransfectedusing2µgpCMVLucand6.4µltransfectionreagent.Luciferaseactivitywasmeasured24hpost-transfection.

Easytouseprotocol

ThejetPEI^®-MacrophageprotocolisassimpleasthejetPEI^®one:MixtheDNAwiththereagenttoformcomplexesandsimplyaddthemixturetothecells.jetPEI^®-Macrophageiscompatiblewithserumandantibiotics,thuseliminatingtheneedformediachange.Proteinexpressionisdetermined24hto72hpost-transfection.

Applications:

jetPEI^®-Macrophageisperfectlysuitedforplasmiddelivery(DNA,shRNAormiRNA)tocellsthatexpressmannose-specificmembranereceptors,suchasprimarymacrophages,glial&dendriticcells.

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Citations:

HereisaselectionofrelevantreferencesusingjetPEI-Macrophage®,moreareavailableinourPolyplus-transfectionDatabase.Markovic,D.S.,Vinnakota,K.,Chirasani,S.,Synowitz,M.,Raguet,H.,Stock,K.,Sliwa,M.,Lehmann,S.,Kalin,R.,vanRooijen,N.,Holmbeck,K.,Heppner,F.L.,Kiwit,J.,Matyash,V.,Lehnardt,S.,Kaminska,B.,Glass,R.,Kettenmann,H.(2009).GliomasinduceandexploitmicroglialMT1-MMPexpressionfortumorexpansion.,ProcNatlAcadSciUSA 106,12530.Pal,R.,BasuThakur,P.,Li,S.,Minard,C.,Rodney,G.G.(2013).Real-timeimagingofNADPHoxidaseactivityinlivingcellsusinganovelfluorescentproteinreporter.,PLoSONE 8,e6398.Wang,Y.,Guo,Y.,Wang,X.,Huang,J.,Shang,J.,Sun,S.(2012).SerumamyloidPcomponentfacilitatesDNAclearanceandinhibitsplasmidtransfection:implicationsforhumanDNAvaccine.,GeneTher 19,70-7.Aflaki,E.,Doddapattar,P.,Radovic,B.,Povoden,S.,Kolb,D.,Vujic,N.,Wegscheider,M.,Koefeler,H.,Hornemann,T.,Graier,W.F.,Malli,R.,Madeo,F.,Kratky,D.(2012).C16ceramideiscrucialfortriacylglycerol-inducedapoptosisinmacrophages.,CellDeathDis 3,e280. Wang,D.,Lou,J.,Ouyang,C.,Chen,W.,Liu,Y.,Liu,X.,Cao,X.,Wang,J.,Lu,L.(2010).Ras-relatedproteinRab10facilitatesTLR4signalingbypromotingreplenishmentofTLR4ontotheplasmamembrane.,ProcNatlAcadSciUSA 107,13806.

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Polyplus Transfection/jetPEI®-Macrophage/Polyplus Transfection-163151/1 Ea