Signosis/Human Obesity ELISA Strip (Colorimetric)/EA1001/1 Ea

Description:

Obesityincreasestheriskforthemetabolicsyndrome,diabetes,hypertension,atherosclerosis,andthrombosis.Anumberofproteinshavebeenidentifiedtobeinvolvedinthedevelopmentoftheobesityrelatedmetabolicsyndrome,diabetes,andcardiovasculardiseases.TheseincludeADIponectin,leptin,TNFa,IGF-1,Resistin,TGFß,IL-6,andPAI-1.PlasmaconcentrationsoftheseproteinsareusuallymeasuredbyELISA.Tosystematicallyexaminetheeffects,SignosisdevelopedanELISAstrip,whichallowssimultaneousdeterminationof8obesitycytokines. Thedifferenceoftheseproteinsbetweentwosamplescanbedeterminedthroughdatacomparisons.Therefore,itfacilitatesthediscoveryofthechangeoftheseproteinsindifferentsamples.

Principle:

Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.

 

 

 

Data: