Signosis/STAT1 ELISA Kit (Colorimetric)/TE0023/1 Ea

Description:

Stat3(signaltransducerandactivatoroftranscription3)promotescellsurvival/proliferation,motilityandimmunetoleranceandisconsideredasanoncogene.Whensignaledbycytokinesandgrowthfactors,suchasoncostate,theactivatedStat3istranslocatedintothenucleus,wherethegeneexpressionisregulatedbybindingtotheDNArecognitionsite.SignosishasdevelopedaStat3ELISAkittodetectStat3activation.STAT1exertsawidespectrumoffunctionsonbothtumorcellsandtheimmunesystem. Stat1isusuallyconsideredasatumorsuppressorbyenhancing inflammationandtriggeringanti-proliferativeandpro-apoptoticresponsesintumorcells.Uponactivatedbycytokines,suchasINFgamma,stat1formshomodimersorheterodimerswithStat3orStat2andbindstotheresponseelementonthepromoterregionoftargetgenes.SignosishasdevelopedaStat1ELISAkittomonitorStat1activation.
 
WhileSTAT3promotescellsurvival/proliferation,motilityandimmunetoleranceandisconsideredasanoncogene,STAT1enhancesinflammationandinnateandadaptiveimmunity,triggeringinmostinstancesanti-proliferativeandpro-apoptoticresponsesintumorcells.Despitebeingactivatedbycommoncytokinesandgrowthfactorreceptorpathways,theiractivationisreciprocallyregulatedtoredirectandbalancecytokine/growthfactorsignalsfromproliferativetoapoptotic,orfrominflammatorytoanti-inflammatory.SignosishasdevelopedStat3/Stat1ELISAkittodistinguishtheactivationof Stat3andStat1.

Principle:

TFELISAkitishighsensitiveandspecificassaywithasimpleandoptimizedprocedure.The96-well(8X12strip)clearplateispre-immobilizedwiththeTFconsensussequencingoligo.TheactivatedTFinnuclearextractorthewholecelllysateisaddedinthewellandbindstotheoligo.TheactivatedTFisdetectedwithaspecificantibodyagainstthesubunitandaHRPconjugatedsecondaryantibody.Theassayutilizescolorimetricdetectionmethod,whichcanbeeasilymeasuredbyspectrophotometry.