Signosis/Apoptosis Luciferase Reporter Vector Set/LR30022000/1 Ea

Description:

Apoptosisisknownasprogrammedcelldeathimplicatedinbiologicalprocessesrangingfromembryogenesistoageingandfromnormaltissuehomoeostasistomanyhumandiseases.Anumberofsignalingpathwaysthatleadtocharacteristiccellchangesareinvolvedinmediatingtheapoptosisresponses.Onecriticalchangeinsignalingpathwaysistheactivationoftranscriptionfactors(TFs),andTF-basedreportersareoftenusedtomonitortheactivationofsignalingpathways.
Signosishasdevelopedasetofluciferasereportervectors(NFkB,p53,Myc,AP1,IRF,ELK,NFAT,andNegativecontrol)tofacilitateapoptosisstudy.

ApplicableGrid:

 

SetsAvailable:(Clickbelowforpricingandordering)

ApoptosisLuciferaseReporterVectorSet(50ng/vector)LR-3002-50

ApoptosisLuciferaseReporterVectorSet(2000ng/vector)LR-3002-2000

Principle:

EithertheTFpromotor(pLuc5U-OxidativeStressLuciferaseReporterVectorSet)ortheTFbindingsite(Allothersets)isupstreamofTATApromotertoinducetheexpressionofluciferasegene.Itresultsintheinductionofluciferaseenzymeactivity.Throughcomparisonofluciferaseactivityintwosamples,eitherTFexpression(pLuc5U-OxidativeStressLuciferaseReporterVectorSet)ortheup-ordown-regulationofTFscanbequantitativelymeasured.

Data: