Signosis/STAT3/STAT1 ELISA Kit (Colorimetric)/TE0025/1 Ea

Description:

Stat3(signaltransducerandactivatoroftranscription3)promotescellsurvival/proliferation,motilityandimmunetoleranceandisconsideredasanoncogene.Whensignaledbycytokinesandgrowthfactors,suchasoncostate,theactivatedStat3istranslocatedintothenucleus,wherethegeneexpressionisregulatedbybindingtotheDNArecognitionsite.SignosishasdevelopedaStat3ELISAkittodetectStat3activation.STAT1exertsawidespectrumoffunctionsonbothtumorcellsandtheimmunesystem. Stat1isusuallyconsideredasatumorsuppressorbyenhancing inflammationandtriggeringanti-proliferativeandpro-apoptoticresponsesintumorcells.Uponactivatedbycytokines,suchasINFgamma,stat1formshomodimersorheterodimerswithStat3orStat2andbindstotheresponseelementonthepromoterregionoftargetgenes.SignosishasdevelopedaStat1ELISAkittomonitorStat1activation.

Principle:

TFELISAkitishighsensitiveandspecificassaywithasimpleandoptimizedprocedure.The96-well(8X12strip)clearplateispre-immobilizedwiththeTFconsensussequencingoligo.TheactivatedTFinnuclearextractorthewholecelllysateisaddedinthewellandbindstotheoligo.TheactivatedTFisdetectedwithaspecificantibodyagainstthesubunitandaHRPconjugatedsecondaryantibody.Theassayutilizescolorimetricdetectionmethod,whichcanbeeasilymeasuredbyspectrophotometry.

 

 

 

Data:

Stat3ELISAanalysis.Helacellsweretreatedwithandwithout10ng/mloncostatinfor1hour,thenthenuclearextractswerepreparedandsubjectedtoStat3ELISAkit. 

 
 
Stat1ELISAanalysis.Helacellsweretreatedwith andwithout10ng/mlIFNgammafor1hour,thenthenuclearextractswerepreparedandsubjectedtoStat1ELISAkit