Signosis/Human Cytokine ELISA Plate Array (Colorimetric)/EA4002/1 Ea

Description:

Cytokinesaresignalingmoleculesthathavecriticalrolesinmanybiologicalprocessessuchascellulargrowth,differentiation,geneexpression,migration,immunity,andinflammation.Cytokinesthataresecretedfromcellsbindtocell-surfacereceptors, initiatetheactivationofsignaltransductionpathwaysandmediatecelltocellcommunication.Themalfunctionofcytokinesleadstomanydiseases,includingarthritis,acuteandchronicliverdisease,inflammatoryboweldisease,cardiac-relateddiseases,andcancers.Cytokinesarecommonlyworkingtogether ina biologicalordiseaseprocess.Therefore,thecomprehensiveanalysisoftheexpressionofmultiplecytokinesallowseffectiverevealingoftheunderneathmechanismofcytokineaction andthealterationleadingto diseases.TheHumanCytokineELISAPlateArrayisacolormetricdetectionthatallowsyoutomonitortheabundanceof31humancytokinessimultaneously.Thisfastandsensitiveassay canbeusedforquantitativecomparisonof thesecytokinesamongdifferent samples.

ApplicableGrid:

ListofApplicableCytokines

1

2

3

4

5

6

7

8

9

10

11

12

A

TNFa

VEGF

PDGF-BB

IL-10

TNFa

VEGF

PDGF-BB

IL-10

TNFa

VEGF

PDGF-BB

IL-10

B

IFNy

EGF

PIGF-1

FGFb

IFNy

EGF

PLGF-1

FGFb

IFNy

EGF

PLGF-1

FGFb

C

G-CSF

IL-6

b-NGF

Leptin

G-CSF

IL-6

b-NGF

Leptin

G-CSF

IL-6

b-NGF

Leptin

D

GM-CSF

Resistin

SCF

IGF-1

GM-CSF

Resistin

SCF

IGF-1

GM-CSF

Resistin

SCF

IGF-1

E

IL-1a

PAI-1

MCP-1

TGF-b

IL-1a

PAI-1

MCP-1

TGF-b

IL-1a

PAI-1

MCP-1

TGF-b

F

IL-8

IL-12

MIP-1a

Adipo

IL-8

IL-12

MIP-1a

Adipo

IL-8

IL-12

MIP-1a

Adipo

G

IP-10

IL-13

IL-2

IL-17a

IP-10

IL-13

IL-2

IL-17a

IP-10

IL-13

IL-2

IL-17a

H

Rantes

Eotaxin-3

IL-4

IL-1b

Rantes

Eotaxin-3

IL-4

IL-1b

Rantes

Eotaxin-3

IL-4

IL-1b

Principle:

The96-wellwhiteplateisdividedinto3or4sections,andeachsectionhas4or3columnsforonesample.Ineachsection,32(HumanCytokineELISAPlateArrays)or24(MouseCytokineELISAPlateArray)specificcytokinecaptureantibodiesarecoatedon32or24wellsrespectively.Thesample,cellculturesupernatants,celllysates,tissuehomogenates,serum,orplasmasamplesamongothers areincubatedwiththecytokineELISAplate,and thecapturedcytokineproteinsaresubsequentlydetectedwithacocktailofbiotinylateddetectionantibodies.Thetestsampleisallowedtoreactwith apairof antibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheplateisfurtherdetectedwithHRPluminescentsubstrateorHRPsubstrateTMB.Thelevelofexpressionforeachspecificcytokineisdirectlyproportionaltotheluminescentorcolor intensity.

Data:

AnalysisofCytokineProteinExpressioninTNFa-TreatedandUntreatedHeLawithHumanCytokineELISAPlateArray.HeLacellswerestarvedfor24hourswithserum-freemedium,subsequentlytreatedthecellswithandwithout20ng/ulTNFfor16hours.Theserum-freeconditionedmediawereincubatedontheplatefor1hour.AfterincubatingwithdetectionantibodymixandHRP,theplatewasdetectedwithTMBsubstratebyaplatereader.