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Schematic of RNAlater™ procedure.
Histology of RNAlater®-preserved tissue.RNAlater®-preserved samples may be used for histolological analysis. Antigenicity is not always preserved; however, some researchers have had success with this technique. The figure shows sections of RNAlater®-preserved material. These sections showed excellent morphological detail and were indistinguishable from untreated samples when examined for standard histological criteria. (A) H&E stained, frozen section of human skin preserved for one week in RNAlater prior to processing. (B) Stained as above but formalin-fixed, paraffin-embedded section of human skin preserved for one week in RNAlater prior to processing. Contributed by Scott Florell and Sancy Leachman, Huntsman Cancer Institute. Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, Jones DA, Leachman SA. (2001) Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol. 14(2):116-128." data-omni-action="View figure">
Histology of RNAlater®-preserved tissue.
Compatibility of various RNA isolation methods with tissue stored in RNAlater™.Freshly dissected whole mouse liver and heart were placed in RNAlater™ and stored at 4°C for three days. RNA was isolated from equal mass amounts of each tissue using the ToTALLY RNA, RNAqueous, or Poly(A)Pure Kits. 5 µg of RNA prepared from each tissue sample was analyzed by denaturing formaldehyde agarose gel electrophoresis (with RNA Millennium Markers)." data-omni-action="View figure">
Compatibility of various RNA isolation methods with tissue stored in RNAlater™.
Ribonuclease protection assay using mouse tissues stored in RNAlater™.Various mouse tissues were stored in RNAlater™ for 1 or 4 weeks at 4°C. RNA was isolated from each tissue and analyzed using the RPA III™ Kit. 10 µg of total RNA was hybridized with 5 x 10<sup>4</sup> cpm of each of 5 combined antisense RNA probes, digested with RNase, and precipitated. Products were assessed on a 5% polyacrylamide/8M urea gel and exposed to film for 4 hr at -80°C with an intensifying screen." data-omni-action="View figure">
Ribonuclease protection assay using mouse tissues stored in RNAlater™.
Northern blot of RNA from tissue treated with RNAlater™.The dissected tissue (0.5 cm<sup>3</sup> fragments or smaller) is submerged in approximately 5 volumes of RNAlater™ (e.g., a 0.5 g sample requires about 2.5 mL of RNAlater) at room temperature. The solution permeates the cells, stabilizing the RNA. The sample can then be stored at room temperature (25°C) for up to a week, at 4°C for up to a month, or at -20°C indefinitely. A northern blot (of gel in the top panel) that was hybridized with probes to p53 and GAPDH, demonstrates the integrity of RNA isolated from tissues treated with RNAlater™ and stored at different temperatures (bottom panel). Note that the tissues include spleen, an organ known for its high RNase content, and from which it is often difficult to extract intact RNA." data-omni-action="View figure">
Northern blot of RNA from tissue treated with RNAlater™.
RNA stability of RNAlater™-treated tissues after multiple freeze/thaw cycles.Mouse heart and liver were dissected and placed in RNAlater™. The tissues were frozen on dry ice and thawed at room temperature for the indicated number of cycles. RNA was isolated using RNAwiz™, electrophoresed on a 1% formaldehyde/agarose gel, ethidium bromide stained, and photographed." data-omni-action="View figure">
RNA stability of RNAlater™-treated tissues after multiple freeze/thaw cycles.
| 描述: | RNAlater™ Stabilization Solution |
|---|---|
| 数量: | 1 x 250mL |
| 外形: | Liquid |
| 试剂类型试剂类型: | RNA Storage Reagent |
| Shipping Condition: | Room Temperature |
| Product Line: | Ambion™, RNAlater® |
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