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eEnzyme/Protease Activity Assay Kit (Red Fluorescence)/CA-P501/1 Ea

CA-P501

Name

EliteTMProteaseActivityAssayKit(RedFluorescence)

Description

Thekitusesaredfluorescentcaseinconjugatethatisproventobeagenericsubstrateforabroadspectrumofproteases(e.g.trypsin,chymotrypsin,Thermolysin,proteinaseK,proteaseXIV,andelastase).Intheintactsubstrate,caseinisheavilylabeledwithafluorescentdye,resultinginsignificantfluorescencequenching.Protease-catalyzedhydrolysisrelievesitsquenchingeffect,yieldingbrightlyfluorescentdye-labeledshortpeptides.

Application

Theassayisidealforroutineproteaseassaysfortheisolationofproteases,orfordetectingproteasescontaminationinsamples.

Size

500assays

Detection

Microplatereader(Ex/Em=540/590nm)

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