产品资料

Agrisera/PR-10 | Pathogenesis-related protein 10/AS13 2724/


productinformation
Background		Pathogenesis-relatedprotein10isproducedinplantsintheeventofapathogenattack.
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromOryzasativaPR-10eprotein, UniProt:Q2QNS9
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50µl
Reconstitution		Forreconstitutionadd50µlofsterilewater
Storage		Theantibodymaybestoredat-20℃foroneyearinitsoriginalformulation.Additionally,antibodymaybestoredat2℃to8℃forupto1monthwithoutdetectablelossofactivity.Avoidrepeatedfreeze-thawcyclesofthedilutedantibody. Storeat4°C;makealiquotstoavoidworkingwithastock.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromliquidmaterialadheringtothecaporsidesofthetubes.
Testedapplications		WesternBlot(WB)
Relatedproducts		AS142810 \|anti-PR-4\|Pathogenesis-relatedprotein4,rabbitantibody AS122369 \|anti-PR-4\|Pathogenesis-relatedprotein4(ArABIdopsisthaliana),rabbitantibody AS152858 \|anti-PR-5\|Pathogenesis-relatedprotein5,rabbitantibody AS122373 \|anti-PR-5\|Pathogenesis-relatedprotein5(Arabidopsisthaliana),rabbitantibody collectionofantibodiestoproteinsinvolvedinpathogenresponse Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:1000(WB)
Expected\|apparentMW		14\|50kDa
Confirmedreactivity		Oryzasativa
Predictedreactivity		Oryzasativa
Notreactivein		noconfirmedexceptionsfrompredictedreactivityknowninthemoment
Additionalinformation
Selectedreferences		tobeaddedwhenavailable,antibodyreleasedinApril2015.

Applicationexample:

TotalproteinfromOryzasativarice(CV.9311)flagleafatthe tilleringstagewasgroundintoafinepowderinliquidnitrogen.An800ulaliquotofextractionbuffer[62.5mMTRIS-HCl(pH7.4),10%glycerol,0.1%SDS,2mMEDTA,1mMphenylmethylsulphonylfluoride(PMSF),5%(v/v)b-mercaptoethanol]wasaddedtoeach300mgpowdersample.Themixturewasvortexedandthenchilledonicefor10min.Sampleswerecentrifugedat12,000rpmfor10min.at4℃,andthesupernatantwascollectedandstoredat–70℃.TheproteinconcentrationsofthericesamplesweredeterminedusingtheBradfordmethod(Bradford,1976).20µgofproteinwasseparatedon12%SDS-PAGEandblotted1htoPVDF.
Blotswereblockedwithfor1hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:1000for1hatRTwithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15minand3timesfor5mininTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorserADIshperoxidaseconjugated)dilutedto1:10000infor1hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor5minwithECLaccordingtothemanufacturer’sinstructions.Exposuretimewas 2min.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Agrisera/PR-10 | Pathogenesis-related protein 10/AS13 2724/