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Kapa biosystems/KAPA RNA HyperPrep Kits with RiboErase (HMR)/KK8710/24 adapters x 40 µl each188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Kapa biosystems/KAPA RNA HyperPrep Kits with RiboErase (HMR)/KK8710/24 adapters x 40 µl each

Description:

KAPA Single-Indexed Adapter Kit, Set A + B (1.5 µM)

KAPA RNA HyperPrep Kits with RiboErase (HMR)

Single-Day RNA

The KAPA RNA HyperPrep Kits with RiboErase (HMR) utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality rRNA-depleted RNA-seq libraries. By utilizing a targeted enzymatic method for depletion, our workflow enables efficient reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). The strand-specific workflow is flexible – supporting library construction from lower-input amounts and degraded samples. Kits contain all reagents required for rRNA depletion and library preparation, with the exception of KAPA Adapters (available separately). Benefits include:

  • single-day library construction, inclusive of rRNA depletion
  • reduced hands-on and overall time through fewer enzymatic and reaction cleanups
  • flexible input of 25 ng – 1 µg total RNA from human, mouse, or rat species*
  • higher success rates with lower input and degraded samples
  • maintain over 99% strand specificity*
  • KAPA Pure Beads included for reaction purifications

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator. Download our Adapter and Bead Calculator

 

*Data on file.
For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

The KAPA RNA Hyper Prep Kit with RiboErase (HMR) results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 25 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
Better utilize sequencing capacity. (B) Libraries were generated in quadruplicate with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
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Sequence what matters

  • Waste fewer reads due to the combination of rRNA carryover and PCR duplicates
  • Identify more unique transcripts and genes with equivalent sequencing
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (A) Libraries were generated with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (B) Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes was demonstrated using the Kapa workflows. Data on file.
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Achieve superior coverage uniformity

  • Obtain more uniform distribution of reads across transcripts
  • Improve coverage of difficult GC-rich transcripts
Identify more genes and transcripts. With equivalent sequencing, the KAPA RNA HyperPrep Kit with RiboErase (HMR) in comparison to competitor workflows identified more genes and transcripts. Data on file.
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Generate high-quality libraries from degraded samples

  • Input as little as 25 ng with FFPE samples, depending on total RNA quality
  • Achieve low duplication rates and highly efficient, reproducible rRNA removal with degraded samples
  • Identify more unique transcripts and genes with equivalent sequencing
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
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Achieve reliable results with degraded inputs

  • Attain a high-degree of expression correlation between paired FFPE and fresh frozen samples which provides increased confidence in sequence data accuracy

Related Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

KAPA mRNA HyperPrep Kits

KAPA mRNA HyperPrep Kits

KAPA Stranded mRNA-Seq Kits

KAPA Stranded mRNA-Seq Kits

Library Amplification

KAPA Library Amplification Kits

Library Preparation

KAPA HyperPrep Kits

Library Quantification

KAPA Library Quantification Kits

Applications:

Applications
  • Whole transcriptome
  • Gene expression analysis
  • Detection of gene fusions, isoforms, and other structural variants
  • Novel transcript identification, including noncoding transcripts
  • SNV discovery

Kit Specifications and Contents / Storage:

Kit Specifications and Contents / Storage

Enzymes and buffers for rRNA depletion, cDNA synthesis, and library preparation can be stored for up to 10 months at -20°C. KAPA Pure Beads can be stored for up to 10 months at 4°C. (US only)

Kits contain Hybridization Buffer, Hybridization Oligos (HMR), Depletion Buffer, RNase H, DNase Buffer, DNase, Fragment, Prime and Elute Buffer (2X), 1stStrand Synthesis Buffer, KAPA Script, 2nd Marking Buffer, 2nd Strand Synthesis & A-Tailing Enzyme Mix, Ligation Buffer, DNA Ligase, PEG/NaCl Solution, KAPA Pure Beads, Library Amplification Primer Mix (10X), and KAPA HiFi HotStart ReadyMix (2X).

RNAHyperRibo_Component Chart

Specifications

Spec
Description
Compatible Platform
0616
Library Type
RNA
Starting Material
High-quality and degraded total RNA
Input Amount
25 ng – 1 µg

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