Kapa biosystems/KAPA Stranded mRNASeq Kits/KK8722/96 adapters x 20 µl each

Description:

Even difficult messages should be understood.

KAPA Stranded mRNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provide precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved coverage of GC-rich and low-abundance transcripts.* Kits contain KAPA HiFi for high-efficiency and low-bias library amplification, as well as KAPA mRNA Capture Beads and a streamlined, “with-bead” protocol.

KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, but do not contain the KAPA mRNA Capture Beads. Kits can be used to prepare libraries from 10 – 400 ng of either poly(A)-selected, ribosomally depleted, or total RNA.

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download Adapter and Bead Calculator

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For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Uncover challenging transcripts 

• Improved coverage of GC-rich transcripts
• Enhanced identification of exonic regions

Detect low-abundance transcripts

• Enables identification of transcripts missed by competitor kits, even with high input*
• High uniformity across varying amounts of sample input*

30M), KAPA Stranded mRNA-Seq libraries generate a greater percentage of mapped reads and lower duplication rates than analogous libraries prepared with the Illumina Stranded mRNA Sample Prep Kit while maintaining 99% strand specificity. Data on file.">

Identify more genes

• Higher percentage of uniquely mapped reads compared to Illumina TruSeq™ Stranded mRNA Sample Prep Kits*
• Lower duplication rates yield better coverage

Maintain high coverage uniformity

• Minimal 5’–3′ bias across transcripts
• More uniform distribution of reads over each transcript

 

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Applications:

Kit Specifications and Contents / Storage: