Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Kapa biosystems/KAPA Hyper Prep Kits/KK8701/12 adapters x 40 µl each188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线

Kapa biosystems/KAPA Hyper Prep Kits/KK8701/12 adapters x 40 µl each

Description:

KAPA Single-Indexed Adapter Kit, Set A (30 µM)

KAPA Hyper Prep Kits

Shift your workflow into hyperdrive.

The KAPA HyperPrep Kit is a versatile, streamlined solution for DNA library preparation for Illumina® sequencing.

The novel one-tube chemistry, contain optimally formulated and evolved enzymes that enable higher yields of adapter-ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our Adapter and Bead Calculator

 

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 μg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 μg of amplified library required for target capture. Data on file.
Number of amplification cycles needed to generate ≥1 µg amplified library for target capture with the SeqCap EZ Comprehensive Cancer Design (578 genes, 4 MB; Roche Nimblegen), for libraries prepared with each kit. The higher conversion rates achieved with the KAPA Hyper Prep Kit, combined with high-efficiency, low-bias amplification with KAPA HiFi DNA Polymerase, translates to significantly less cycles of pre-capture amplification and subsequent improvements in sequence data quality. Data on file.
Prev Next

Improve library yields and sequence quality

  • Higher library yields translate to greater molecular complexity
  • Fewer cycles of amplification with KAPA HiFi DNA Polymerase results in lower duplication rates and improved coverage
Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage. Data on file.
Conversion rates for FFPE DNA has been identified as a primary bottleneck in NGS library construction. With the KAPA Hyper Prep Kit, conversion rates for FFPE samples are similar to those for high-quality DNA. This leads to significantly lower duplication rates and higher overall target coverage in exome and other target capture workflows. Conversion data are for libraries prepared from 80 – 100 ng. Covaris-sheared FFPE DNA of variable quality, or 100 ng control DNA. Data on file.
Prev Next

Create high-quality libraries from FFPE samples

  • Generate high-quality libraries from 250 ng FFPE DNA or less*
  • Significantly lower duplication rates and higher coverage*
The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing. Data on file.

Complete library construction in less than 3 hours

  • One-tube workflow with minimal cleanup steps reduces overall time, and minimizes hands-on time
  • Sample-to-library in <2 hours for PCR-free workflows, or <3 hours with amplification*
  • Fewer handling steps lead to improved consistency and reproducibility
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to reduce duplication rates. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
Prev Next

Improve performance with low-input samples

  • Generate more sequence-quality library molecules without increasing adapter-dimers
  • Increase yields without inducing size bias

 

*Data on file.

Related Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

Sample QC

KAPA hgDNA Quantification and QC Kit

Library Amplification

KAPA Library Amplification Kits

Library Quantification

KAPA Library Quantification Kits

KAPA Stranded mRNA-Seq Kits

KAPA Stranded mRNA-Seq Kits

Applications:

Applications
  • Whole Genome Sequencing
  • Whole Exome Sequencing
  • Targeted Sequencing (custom panels)
  • ChIP-seq
  • Amplicon Sequencing
  • Methyl-seq

Kit Specifications and Contents / Storage:

Kit Specifications and Contents / Storage

Kits can be stored for up to 12 months at -20˚C.

Kits contain KAPA End Repair & A-Tailing Buffer, KAPA End Repair & A-Tailing Enzyme, KAPA Ligation Buffer, KAPA DNA Ligase, KAPA HiFi HotStart ReadyMix and KAPA Library Amplification Primer Mix

Components

Hyper_Component Chart

Specifications

Spec
Description
Compatible Platform
llumina HiSeq, MiSeq, NextSeq and GAIIx
Library Type
DNA
Starting Material
Fragmented DNA, cDNA, or PCR amplicons
Input Amount
1 ng–1 µg

新闻动态
行业前沿
技术文章
最新产品

188进口试剂采购网 www.188bio.cn -中国试剂网,试剂网,化学试剂网,国药试剂,抗体公司,试剂公司,试剂盒公司,苏州试剂公司,北京化学试剂公司,天津化学试剂,试剂商城,试剂代理,流式抗体 细胞库查询 sitemap