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Kapa biosystems/KAPA HyperPlus Kits/KK8001/30 mL

Description:

KAPA Pure Beads (30 mL)

KAPA HyperPlus Kits

NGS library preparation.  Evolved.

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube. Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows. Benefits include:

  • DNA fragmentation and library prep in 2.5 hours*
  • Flexible DNA sample input from 1 ng – 1 µg*
  • Reduced bias and maximize sequence coverage*
  • PCR-free workflows

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our Adapter and Bead Calculator

 

*Data on file.
For Research Use Only. Not for use in diagnostic procedures.


Workflow

KAPA HyperPlus Workflow

Product Highlights

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Tunable and reproducible fragmentation

  • Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
  • Reproducible insert sizes across a range of GC content and DNA input amounts

Industry leading library yields…

  • Routinely achieve conversion rates >80% from ≥ 100 ng input*
  • Superior performance across a range of DNA input amounts*
  • High library yields enable PCR-free workflows from as little as 50 ng starting material*
Single nucleotide polymorphism (SNP) detection sensitivity indicates better sequencing quality. Libraries were prepared for capture using the Nimblegen SeqCap EZ HGSC VCRome panel, 50 ng input, standard capture protocol. SNPs were called using LoFreq with default settings and a minimum per-base depth of coverage cut-off of 10. Sensitivity (true positive rate) was calculated as the ratio of true positives divided by the sum of true positives and false negatives. Total SNPs analysed was greater than 25,000. Data on file.
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…Enable superior sequencing results

  • Higher conversion rates results in fewer amplification cycles and lower duplication rates
  • Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
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Minimal sequence coverage bias

  • Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods*
  • Equivalent performance to mechanical shearing*
  • Less bias leads to more uniform sequencing coverage and reduced sequencing costs*

 

*Data on file.

Related Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

Sample QC

KAPA hgDNA Quantification and QC Kit

Library Amplification

KAPA Library Amplification Kits

Library Quantification

KAPA Library Quantification Kits

KAPA Stranded mRNA-Seq Kits

KAPA Stranded mRNA-Seq Kits

Applications:

Applications
  • Whole Genome Sequencing
  • Whole Exome Sequencing
  • Targeted Sequencing (custom panels)
  • FFPE Sample Sequencing

Kit Specifications and Contents / Storage:

Kit Specifications and Contents / Storage

Kits can be stored for up to 6 months at -20ºC.

Kits contain KAPA Frag Enzyme, KAPA Frag Buffer (10X), KAPA Frag Conditioning Solution, KAPA End Repair & A-Tailing Buffer, KAPA End Repair & A-Tailing Enzyme, KAPA Ligation Buffer, KAPA DNA Ligase, KAPA HiFi HotStartReadyMix and KAPA Library Amplification Primer Mix.

 

 

Specifications

Spec
Description
Compatible Platform
llumina HiSeq®, MiSeq®, NextSeq®
Library Type
DNA
Starting Material
genomic DNA, FFPE DNA, PCR amplicons
Input Amount
1 ng–1 µg

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