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Biohelix/E. coli UvrD Helicase/H0260S/20 µg

Description:

  


E.coliUvrDisaDNAhelicasethatusestheenergygeneratedbythehydrolysisofnucleosidetriphosphatestobreakthehydrogenbondslinkingthetwostrandstogetherinduplexDNA.Itisactiveat37ºC.

SUPPLIEDWITH:
10XHelicaseBufferM


SuppliedbyUsers:ATP

      

Source:

  

PurifiedfromanE.colistrainthatoverexpressesE.coliuvrDgene.      

Reactioncondition:

  


1XHelicaseBufferM:
10mMDithiolthreitol
10mMMagnesiumAcetate
35mMTris-AcetatepH7.5@25°C

3mMATP.
Incubateat37ºC.

Notes:
E.coliUvrDhelicasemayrequireMutLand/orSSBforoptimalperformanceinspecificapplications.

:MutLstimulatesUvrDhelicaseactivitymorethantenfoldbyloADIngitontotheDNAsubstrate(MechanicLE,FrankelBA,MatsonSW(2000)JBiolChem275:38337–38346).

:MutLandSSBarerequiredinHelicase-DependentAmplificationusingE.coliUvrD(MyriamVincent,YanXu,andHuiminKong(2004)EMBOreportsVol.5,795-800).

    

StorageConditions:

  


StorageTemperature:
-20ºC

Concentration:
0.5mg/ml

StorageBuffer:
20mMTris-HClpH8.3
200mMNaCl
1.5mMEDTA
25mMß-Mercaptoethanol
50%Glycerol

    

QualityControl:

  


QualityAssuranceStatement:
E.coliUvrDHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivity.

            
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