...Cleaved, N-terminus FFGVG neoepitopes, clone AF-28; 100 μg...

Species ReactivityKey ApplicationsHostFormatAntibody Type B, H, M, Po, R, not Eq, not Gp ELISA, WBMousePurifiedMonoclonal Antibody Description: Anti-Aggrecan, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 Background Information: Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces. Clone: AF-28 Specificity: Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases. Key Applications: ELISA Western Blotting Application Notes: Western blot

ELISA

Optimal working dilutions must be determined by the end user. Species Reactivity: Bovine Human Mouse Pig Rat Non-Reactive Species: Horse Guinea Pig Isotype: IgG1 Immunogen: FFGVGGEEDC-KLH peptide Epitope: N-terminus FFGVG neoepitopes Control: Cartilage, neural tube, and brain tissue Presentation: Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative. Storage Conditions: Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Gene Symbol: ACAN MSK16 SEDK CSPCP AGCAN AGC1 CSPG1 CSPGCP Antibody Category: Cell Structure Antibody Sub-Category: ECM Proteins Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Trade Name: Chemicon (Millipore) Format: Purified Host: Mouse Antibody Type: Monoclonal Antibody
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FFGVGGEEDC-KLH peptide 详细描述见链接：http://www.millipore.com/catalogue/item/MAB19310

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