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OptiGene/GspM3.0 LF DNA Polymerase/GspM3.0 DNA polymerase @ 8u/µl/8000u/GSPM3-002188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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OptiGene/GspM3.0 LF DNA Polymerase/GspM3.0 DNA polymerase @ 8u/µl/8000u/GSPM3-002

GspM3.0 LF DNA Polymerase

  • OptiGene offer GspM3.0 DNA Polymerase as a direct replacement for Bst DNA polymerase.

  • GspM3.0 DNA Polymerase has been engineered by in-silico design to offer amplification speed unachievable by alternative suppliers of Bst-like Polymerases.

  • This enzyme enables a direct replacement for existing users of Bst LF DNA Polymerase, yet offers superior amplification speed.

  • Although fast, this enzyme does not offer the enhanced reverse transcriptase activity of GspSSD2.0 DNA Polymerase.

    • Key features:

  • Directly replaces Bst DNA polymerases offering the fastest amplification.

  • Superior strand-displacement activity

  • 65ºC temperature optimum

  • 5’-3’ exonuclease minus

  • 3’-5’ exonuclease minus

  • Supplied with 10X iBuffer5
    • Cat. No:Description
    GSPM3-0011600uGspM3.0 DNA polymerase @ 8u/µl
    GSPM3-0028000uGspM3.0 DNA polymerase @ 8u/µl
    GSPM3-002HC8000uGspM3.0 DNA polymerase @ 100u/µl
    GSPM3-003HC40000uGspM3.0 DNA polymerase @ 100u/µl
    iBuffer2-0012 x 1.5ml iBuffer 2
    iBuffer2-00215ml iBuffer 2
    LAMP (Human gDNA template): Time-to-threshold comparison:GspM3.0 vs competitor ‘N’ (LAMP Pol 3.0) and competitor ‘L’ (thermostable LAMP Pol)

    gspm3

    25µl LAMP reactions: 8U DNA Pol, 1X manufacturers supplied reaction buffer (‘GspM3.0’ – iBuffer5, ‘N’ – IsoPol II, ‘L’ – BufferC), ), dNTPs/MgSO4 and Betaine – as per manufacturers recommended concentrations, 100-10,000 copies Human gDNA (Sigma), 1X Human VKORC1 primer mix – designed using LAMPDesigner™ (0.8µM FIP 5’-CTGTTGCACCTACCCTTCTGGAGCAGGAGAG GGAAATATCA-3’, 0.8µM BIP 5’-GAAGTCAAGCAAGAGAAGACCT GAAAACTCCTGACCTCAAGTGA-3’, 0.2µM F3 5’-ACCATTATTCTGTCTACCACAC-3’, 0.2µM B3 5’-GACAGGGTTTCACCATGTT-3’, 0.4µM LoopF 5’-TCTCTTCCTCTGGCGTCT-3’, 0.4µM LoopB 5’-CTCACGCCTATAATCCTAGCATT-3’), 3.75ng fluorescent intercalating dye (V13-01184 Dyomics GmbH).‘GspM3.0’, ‘N’ and ‘L’ reactions were run as per manufacturer’s instructions at 65ºC, 65ºC and 70ºC respectively on a Genie®II.A: Real-time amplification plots. B: Time-to-threshold values (the maximal rate of change in fluorescence over time) reported in graphical format for simple comparison.

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