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Protein Assay (Spectrophotometer)188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Protein Assay (Spectrophotometer)

ProteinAssay(Spectrophotometer)

  • UseBSA(bovineserumalbumin)1mg/mlstocksolution(1mlEppendorftubes)forstandardcurve.
  • Place0,2,5,10,15,20µg(=µl)BSAintoplasticcuvette.Use2-5µlofsample.
  • Bringvolumeupto800µlwithH2O.
  • Add200µldye(BioradProteinAssayDyeReagentConcentrate)
  • CovercuvetteswithParafilmandmixgently.Thecolourofsample(intensityofblue)shouldbebetweenthetwoextremesofthestandardcurve.Avoidprecipitationbyaddingproteinfirst,H2Osecondanddyelast.
  • Measureproteinconcentrationwithspectrophotometerat595nm


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