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Progen/anti-p21 Protein mouse monoclonal, WA-1, supernatant/16081/1 Ea

p21 immunostaining is most often used in combination with p53 to confirm the presence of a p53 mutation. p53 overexpression in combination with low or absent p21 expression is consistent with a p53 mutation.
Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone WA-1
Immunogen Rec. WAF-1/p21 protein
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (most mammals)
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent
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