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ProteoChem/Iodoacetyl-LC-Biotin 100 mg/b2108/100mg

Iodoacetyl-LC-Biotin(N-Iodoacetyl-N"-BiotinylhexylenediamineorN-Biotinyl-N"-(iodoacetyl)-1,6-hexanediamine)reactswithsulfhydrylgroupstoformstablethioetherlinkages.Iodoacetyl-LC-Biotinhasanextendedspacerarmthatreducessterichindrance.Iodoacetyl-LC-Biotinmustbedissolvedinanorganicsolvent,suchasDMSOorDMF,thenaddedtoanaqueousbiotinylationreaction.Iodoacetyl-LC-BiotincouplingreactionsaremostefficientwhenreactedatatpH7.5-8.5inreducedlight.TheresultingbiotinylatedproteincanthenbedetectedwhenusedinconjunctionwithstreptavidinorImmobilizedStreptavidin.
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