Stemgent血细胞重编程试剂盒正式上市

ReproCELL旗下的血细胞重编程试剂盒，是全球第一个商业化的使用srRNA和micro RNA进行细胞重编程的试剂盒，为干细胞研究人员提供安全、灵活、快速的方法，使外周血、脐带血以及成人和新生儿的成纤维细胞中的内皮祖细胞（EPCs）重编程为iPS细胞。
　这个试剂盒主要是有三部分组成：OKSiM-srRNA，microRNA Reprogramming Cocktail和B18R重组蛋白。

ProductOverview

ReproCELL"sStemgent®StemRNA™-SRReprogrammingKitisthefirstcommercialcellularreprogrammingkitcombiningself-replicativeRNA(srRNA)andmicroRNAtechnologiestoprovidestemcellresearcherswithsafe,flexIBLe,fastandcost-effectivereprogrammingofbothendothelialProgenitorcells(EPCs)derivedfromhumanperipheralandcordbloodaswellasfromhumanadultandneonatalfibroblasts.

Thekitiscomprisedofonevialeachofthreecomponents:OKSiM-srRNA,microRNAReprogrammingCocktailandB18RRecombinantProtein.

Featuresinclude:

Robust,validatedprotocolforreprogramminggeneticallystableEPCsderivedfromhumanperipheralorcordblood
Virus-andDNA-freeOKSiMreprogrammingfactorsusingself-replicativeRNA
Two-transfectionprotocoleliminatesneedforconditionedmedium,co-transfections,smallmoleculesandfeedercells
iPScellcoloniesgeneratedfromEPCsandfibroblastsinasfewas25days
Protocolaccomodates5reprogrammingwellsina6-wellplateformat

TABLE1.EfficientEstablishmentandCellularsrRNAReprogrammingofEPCsDerivedfromHumanBloodSamples

PrimaryEPCestablishmentefficiencyforperipheralbloodiscumulativeofbothfreshbloodandcryopreservedmononuclearcell(MNC)samples.CordbloodefficiencydataarefromcryopreservedMNCsonly.Eachcollagen-coatedT-75flaskwasseededwithaminimumof5x107MNCs.TheunmodifiedStemRNA-SRkitprotocoldemonostratedhighpatient-to-patientefficiencyusingasinglereprogrammingattempt.Typicalfinalyieldsare10-20iPScellcoloniesperwellina6-wellplateformat.

ProductSpecifications

Size

1kit

KitContents

OKSiM-srRNA,(PartNo.05-0038),5µg,1vial
microRNAReprogrammingCocktail(20µM)(PartNo.05-0036),35µL,1vial
B18RRecombinantProtein,Carrier-Free(Cat.No.03-0017),50µg,1vial

StorageandStABIlity

Storeallthreekitcomponentsatorbelow-70°C.
Kitcomponentsarestableforaminimumof3monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

TheStemRNA™-SRReprogrammingKitisfunctionallyvalidatedforsuccessfulRNA-basedreprogrammingofhumanumbilicalveinendothelialcells(HUVECs).CompletereprogrammingofiPScellcoloniesisconfirmedbyexpressionofpluripotencyMarkersandappropriatecolonymorphology.Allcomponentsofthekitaresterileandhavetestednegativeformycoplasma.

LimitedUseLicenses

LimitedUseLicenseMIT

LimitedUseLicenseiPSAcademiaJapan

ThisproductalsousestechnologylicensedfromtheUniversityofCalifornia-SanDiego.

RecommendedUsage

ForusewithassociatedStemRNA-SRprotocolsforEPCderivationandgenerationofiPScells.

References

1、YoshiokaN;GrosE;LiH-R;KumarS;DeaconDC;MaronC;MuortriAR;ChiNC;FuX;YuBD;DowdySF."EfficientGenerationofHumaniPSCsbyaSyntheticSelf-ReplicativeRNA."CellStemCell13:246(2013)

2、GetiI;OrmistonM:TouhainF;ToshnerM;MovassaghM;NicholsJ;MansfieldW;SouthwoodM;BradleyA;RanaAA;VallierL;MorrellNW."Apracticalandefficientcellularsubstrateforthegenerationofinducedpluripotentstemcells."StemCellsTransmed1:855(2012)

3、YoshidaY;TakahashiK;IshisakaT;YamanakaS."HypoxiaEnhancestheGenerationofInducedPluripotentStemCells."CellStemCell5:237(2009)

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Stemgent血细胞重编程试剂盒正式上市