产品资料

Stemgent/KLF4 Antibody | Kruppel-like factor 4/09-0021/100 μl

ImmunocytochemistryanalysisofKlf4onNIH3T3cellstransfectedwithamouseKlf4expressionconstruct(left)andHeLacellstransfectedwithahumanKlf4expressionconstruct(right).TwodaysposttransfectioncellswerestainedwithKlf4Antibody(Purified)ata1:100dilutionfollowedbyasecondaryCy™3antibody(red).DAPIstainingwasperformedtovisualizenuclei(blue).

ProductOverview

ThemammalianKrüppel-liketranscriptionfactor4(Klf4)isanevolutionarilyconservedzincfinger-containingtranscriptionfactorwithdiverseregulatoryfunctionsincellgrowth,proliferation,differentiationandembryogenesis.ItalsoplaysasignificantroleintheprocessoftumOrigenesis.Klf4isoneofthekeytranscriptionfactorsthathavebeenusedtoreprogrammouseandhumanfibroblaststoinducedpluripotentstem(iPS)cells.

ProductSpecifications

Size

100μl

Concentration

0.25mg/ml

Clone

56CT5.1.6

Isotype

MouseIgG1,κ

Immunogen

HumanKlf4recombinantprotein

Reactivity

Mouse,Human

Preparation

Thisantibodywaspurifiedbyaffinitychromatography.

Formulation

Phosphate-bufferedsolution,pH7.2,0.1%sodiumazide,and0.2%BSA

StorageandStABIlity

Storeat4˚Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistrytoensureproductquality.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.Foranapplicationspecificprotocol,pleasereferenceProtocol:Immunocytochemistryonlineatwww.Stemgent.com/support/protocols.

SpecificationSheets

09-0021SpecificationsSheet

SafetyDataSheets

09-0021SafetyDataSheet

Protocols

Protocol:Immunocytochemistry

References

Chen,X.,Whitney,E.M.,Gao,S.Y.,andYang,V.W.(2003)TranscriptionalprofilingofKrüppel-likefactor4revealsafunctionincellcycleregulationandepithelialdifferentiation.JMolBiol.326:665-677.
Ghaleb,A.M.,Nandan,M.O.,Chanchevalap,S.,Dalton,W.B.,Hisamuddin,I.M.,andYang,V.W.(2005)Krüppel-likefactors4and5:theyinandyangregulatorsofcellularproliferation.CellRes.15:92-96.
Takahashi,K.,andYamanaka,S.(2006).Inductionofpluripotentstemcellsfrommouseembryonicandadultfibroblastculturesbydefinedfactors.Cell126,663-676.
Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
Park,I.H.,Arora,N.,Huo,H.,Maherali,N.,Ahfeldt,T.,Shimamura,A.,Lensch,M.W.,Cowan,C.,Hochedlinger,K.,andDaley,G.Q.(2008)Disease-specificinducedpluripotentstemcells.Cell134:877-886.

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