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Stemgent/StainAlive SSEA-4 Antibody (DyLight 550) | Stage-specific Embryonic Antigen 4/09-0097/100 μl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Stemgent/StainAlive SSEA-4 Antibody (DyLight 550) | Stage-specific Embryonic Antigen 4/09-0097/100 μl

09-0097_SA_DL_Ma-M
ICCanalysisofSSEA-4AntibodyonH1humanEScellsat10Xmagnification.[A]PhasecontrastviewofhEScellcolonyinculture,[B]samefieldofviewafterstainingwithStainAliveSSEA-1Antibody(DyLight550)usinga1:100dilution.

ProductOverview

Stage-specificembryonicantigen-4(SSEA-4)isexpressedinavarietyofhumancelllinesincludingembryoniccarcinoma(EC),embryonicstem(ES),andinducedpluripotentstem(iPS)cells.SSEA-4isdown-regulatedupondifferentiation,makingitawidelyusedMarkertocharacterizehumanEScellsandmonitortheirdifferentiation.Inmouse,SSEA-4isexpressedonoocytesandearlycleavage-stageembryos,butnotonECorEScells.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andexpressionofpluripotencymarkersdoesnotchangeafterstainingwiththisantibody.


ProductSpecifications

Size

100μl

Concentration

0.5mg/ml

Clone

MC-813-70

Isotype

MouseIgG3,κ

Immunogen

Humanembryonalcarcinomacellline2102Ep

Reactivity

Human

Preparation

ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight550underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.Theformulationis0.2µmfiltersterilizedandfreefrombacteria,fungi,mycoplasma,andvirus.

Formulation

Phosphate-bufferedsolution,pH7.2,containingnopreservative

StorageandStABIlity

Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistrytoensureproductquality.Endotoxinlevelislessthan0.1EU/µg(0.01ng/µg)oftheproteinasdeterminedbytheLALassay.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.


SpecificationSheets

  • 09-0097SpecificationsSheet

SafetyDataSheets

  • 09-0097SafetyDataSheet

Protocols

  • GeneralProtocolforICCStainingofLiveCells
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