TACS•XL®kitsare basedontheincorporationofbromodeoxyuridine(BrdU)atthe3’OHendsoftheDNAfragmentsthatareformedduringapoptosis.TheincorporationofBrdUbyTdTismoreefficientthaneitherbiotinylatedordigoxigeninlabelednucleotidesusedinotherterminaldeoxynucleotidyltransferasedUTPnickendlabeling(TUNEL)-basedassays.Thedetectionsystemutilizesabiotinconjugatedanti-BrdUantibodyandstreptavidin-horserADIshperoxidase.Thecombinationofantibodyspecificitywiththesignalenhancingpropertiesofbiotinstreptavidinresultsinprecisecellularlabelingandthehighestsignal-to-noiseratioobservedincompetitivetesting.
Pleaserefertoourcomponentslistingfordetails.
Catalog#4828-30-Rincludes: | ||
CatalogNumber | Description | Qty |
4800-30-01 | ProteinaseK | 1 |
4800-30-06 | Streptavidin-HRP | 1 |
4810-30-05 | TdTEnzyme | 1 |
4828-30-04 | B-dNTPMix | 1 |
4828-30-06 | anti-BrdUantibody | 1 |
4828-30-12 | Strep-Diluent | 1 |
4876-05-01 | Cytonin™ | 1 |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn