Expedeon/ELISA-ONE™ for ERK 1/2/ELISA-ONE™ for total ERK 1/2, 1 x 96 Well Plate Kit/ELK005

ELISA-ONE for Phospho ERK 1/2 (pT202/pY204)

Specificity

ELISA-ONE for phospho ERK 1/2 (pT202/pY204) are ready to use ELISA kits for the detection of endogenous levels of ERK 1/2 when phosphorylated at Thr202 and Tyr204.

ELISA-ONE for phospho ERK 1/2 (pT202/Y204) is comprised of a precoated 96 well (12×8 strips) plate and an antibody pair, recognizing the phospho-Thr202 epitope and the phospho-Tyr204 epitope of ERK 1/2.

The antibody pair recognizes human and mouse ERK 1/2 and it is expected to react with rat. However, other species should be tested on a case by case basis.

ELISA-ONE for Total ERK 1/2

Specificity

ELISA-ONE for total ERK 1/2 are ready to use ELISA kits for the detection of endogenous levels of ERK 1/2 irrespective of phosphorylation status.

ELISA-ONE for total ERK 1/2 is comprised of a precoated 96 well (12×8 strips) plate and an antibody pair, recognizing two invariants epitopes of ERK 1/2.

The antibody pair recognizes human and mouse ERK 1/2 and it is expected to react with rat. However, other species should be tested on a case by case basis.

ELISA-ONE for Phospho & Total ERK 1/2

Specificity

ELISA-ONE for phospho and total ERK 1/2 are ready to use ELISA kits for the detection of endogenous levels of ERK 1/2 in its phosphorylated and unphosphorylated form.

ELISA-ONE for phospho and total ERK 1/2 is comprised of a precoated 96 well (12×8 strips) plate and two antibody pairs, recognizing the phospho-Thr202 and phospho-Tyr204 epitopes and an invariant epitope of ERK 1/2.

The antibody pairs recognize human and mouse ERK 1/2 and it is expected to react with rat. However, other species should be tested on a case by case basis.

Technical Specifications

Species Cross-reactivity

Tested: Human, mouse

Expected: Rat

Kit Contents

• 1x 96 well (12×8 strips) CaptSure assay plate
• 1x adherent plate seal
• 1x Lysis Buffer (5X)
• 1x Enhancer Solution
• 1x Wash Buffer (10X)
• 1x Detection Reagent
• 1x Stop Solution
• 1x Lyophilized Positive Control Lysate
• 1x Capture Antibody Reagent 1
• 1x Capture Antibody Reagent 2 (phospho / total target kits only)
• 1x Detection Antibody Reagent 1
• 1x Detection Antibody Reagent 2 (phospho / total target kits only).

ERK Signaling Pathway

ERK 1 and ERK 2, belong to a conserved family of serine / threonine protein kinases. ERK 1/2 form part of a classical MAPK signaling cascade, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK).

ERK 1/2 are involved in cellular signaling events associated with a range of stimuli, including GPCRs, growth factors, and cytokines. The kinase activity of ERK proteins is regulated by dual phosphorylation at Thr202/Tyr204 in ERK 1, and Thr185/Tyr187 in ERK 2. MEK 1 and MEK 2 are the primary upstream kinases responsible for ERK 1/2 phosphorylation in this pathway. Several targets of ERK 1/2 have been identified, including downstream kinases and transcription factors.

ERK References

Roskoski, R Jr. ERK1/2 MAP kinases: structure, function, and regulation (Review). Pharmacol Res. 2012 Aug;66(2):105–-43. doi: 10.1016/j.phrs.2012.04.005. Epub 2012 Apr 27.

Werry, TD., Christopoulos,A., Sexton, PM. Mechanisms of ERK1/2 regulation by seven-transmembrane-domain receptors (Review). Curr Pharm Des. 2006;12(14):1683–702.

Werry, TD., Sexton, PM., Christopoulos, A. “Ins and outs” of seven-transmembrane receptor signalling to ERK (Review). Trends Endocrinol Metab. 2005 Jan–Feb;16(1):26–33.

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