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glycobiotech/Monoclonal antibody against ceramide/N/A

Monoclonalantibodyagainstceramide

ThismousemonoclonalIgMantibodyishighlyspecificforceramideinimmuno-overlaysofthinlayer-chromatography,inimmunohistochemistryandimmuno-electronmicroscopy

Specificity


Fromlefttoright: ceramide-1,ceramide-2,ceramide-3,ceramide-5,ceramideC14,ceramideC16,dihydro-ceramideC16,sphingosine,dihydro-sphingosine,glucosylceramid-2,glucosylceramid-3,galactosylceramid-1,galactosylceramid-2,lactosylceramid,cholesterol,sphingomyelin,phosphatidylcholine,phosphatidylserine,phosphatidylethanolamine

 

Sensitivity



Thinlayerchromatographyofvariousceramidespeciesanddetectionbyconventionalsprayingandbyimmuno-overlaywithmab_0011.Chol=cholesterol

Sensitivityindot-blot

Sensitivity in dot-blot

Simultaneousdetectionofceramidesandcerebrosidesinhumanskin



Doublelabellingofhumanskinwithmonoclonalanti-ceramideantibody (red)
andpolyclonalrabbitanti-glucosylceramide (green)

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