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NewEast/Rac1 Protein [10101][100 µg]/10101

CatalogNumber:  10101
ProductName:  Rac1Protein
Synonyms:  Ras-relatedC3botulinumtoxinsubstrate1,Rac-1,p21-Rac1,MIG5,TC-25
Source:  Human,recombinantfulllength,His6-tag
ExpressionHost:  E.coli
MolecularWeight:  21kDa
Purity:  >95%bySDS-PAGE
Introduction:  SmallGTPasesareasuper-familyofcellularsignalingregulators.RacbelongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofRac,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs).
AminoAcidSequence  (1-192)
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRL RPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKL TPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Properties
PhysicalAppearance(form):  Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.
PhysicalAppearance(form):  Whiteorclear
Concentration:  1mg/mL
Storage:  -80°C
PreparationInstructions:  Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedRac1wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining.
References:  1.AznarBenitah,S.etal.,Science309:933-935,2005.2.Chuang,Y.etal.,CancerRes.64:8271-8275,2004.3.Halet,G.etal.,Dev.Cell12:309-317,2007.4.Machacek,M.etal.,Nature461:99-103,2009.5.Matos,P.etal.,Biochem.Biophys.Res.Commun.277:741-751,2000.6.Moore,K.A.etal.,Biochem.J.326:17-20,1997.7.Walmsley,M.J.etal.,Science302:459-462,2003.8.Wu,Y.I.etal.,Nature461:104-108,2009.
Datasheet:  
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Publications:
1.  Phosphorylationofα-tubulinbyproteinkinaseCstimulatesmicrotubuledynamicsinhumanbreastcellsCytoskeletonVolume71,Issue4,pages257–272,April2014
2.  ImmunofluorescenceandConfocalMicroscopyofNeutrophilsNeutrophilMethodsandProtocolsMethodsinMolecularBIOLOGyVolume1124,2014,pp251-268
3.  Alinkbetweenthenuclear-localizedsrGAP3andtheSWI/SNFchromatinremodelerBrg1MolecularandCellularNeuroscienceVolume60,May2014,Pages10–25
4.  SHP2PhosphatasePromotesMastCellChemotaxistowardStemCellFactorviaEnhancingActivationoftheLyn/Vav/RacSignalingAxisTheJournalofImmunologyApril14,20141301155
5.  ROBO1,atumorsuppressorandcriticalmolecularbarrierforlocalizedtumorcellstoacquireinvasivephenotype:StudyinAfrican-AmericanandCaucasianprostatecancermodelsInternationalJournalofCancerVolume135,Issue11,pages2493–2506,1December2014
6.  TranscriptionfactorsNRF2andnf-kbarecoordinatedeffectorsoftheRHOfamily,GTPbindingproteinrac1duringinflammationJBiolChem.2014May30;289(22):15244-58
7.  CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamicstoPromoteMacrophagePhagocytosisofZymosanMolCellBiol.2014Nov15;34(22):4186-99
8.  p53DownRegulatesPDGF-InducedFormationofCircularDorsalRufflesinRatAorticSmoothMuscleCellsPLoSOne.2014Sep23;9(9):e108257
9.  LPA-mediatedmigrationofovariancancercellsinvolvestranslocalizationofGαi2toinvadopodiaandassociationwithSrcandβ-pixCancerLett.2015Jan28;356(2PtB):382-91
10.  cAMPcontrolstherestorationofendothelialbarrierfunctionafterthrombin-inducedhyperpermeabilityviaRac1activationPhysiologicalReportsPublished24October2014Vol.2no.e12175DOI:10.14814/phy2.12175
11.  CisplatinatSub-toxicLevelsMediatesIntegrinSwitchinLungCancerCellsAnticancerResearchDecember2014vol.34no.127111-7117
12.  Semaphorin-3FsuppressesthestemnessofcolorectalcancercellsbyinactivatingRac1Cancerletters.March1,2015Volume358,Issue1,Pages76–84.
13.  GeometrysensingthroughPOR1regulatesRac1activitycontrollingearlyosteoblastdifferentiationinresponsetonanofiberdiameterIntegrBiol(Camb).2014Dec24
14.  InhibitionofRac1ActivityintheHippocampusImpairstheForgettingofContextualFearMemoryMolNeurobiol.2015Jan24