PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript RT can be used to perform reverse transcription from any RNA template, including GC-rich RNAs and those with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H-minus. Because of the excellent extension capability of PrimeScript RT, preparation of cDNAcan be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase was referenced in a variety of scientific applications such as gene expression profiling, gene discovery, transcriptome analysis, miRNA expression and regulation analysis, molecular evolution studies, virology, and microbiology.
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