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Synaptic Systems/Bassoon/141002/200µl

Polyclonalrabbitantiserum,antibody1
Cat. No. 141 002
crude antiserum
200µlantiserum,lyophilized.Forreconstitutionadd200 µlH2O,thenaliquotandstoreat-20°Cuntiluse.
ApplicationsWB:1:100upto1:1000(APstaining;seeremarks)blot
IP:nottestedyet
ICC:1:200upto1:2000image
IHC:yes
ELISA:nottestedyet
ImmunogenRecombinantproteincontainingthe330C-terminalaaofratbassoon.
ReactivityRat,mouse,nosignalinchicken;otherspeciesnottestedyet.
SpecificitySpecificforbassoon.
Remarks
  • WB:Duetoitslargesize,bassoonrequiresspecialgel-electrophoresisandWesternblotprotocolsforvisualizationbyimmunoblotting.Excellentresultscanbeobtainedwiththe4-12%TRIS-glycinegrADIentgelsofanamed.
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