产品资料

Abcam/Human Fibronectin ELISA Kit (ab219046)/1 x 96 tests/ab219046

Product name
Human Fibronectin ELISA KitSee all Fibronectin kits
Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Serum 3 5%
Inter-assay
Sample n Mean SD CV%
Serum 5 8.3%
Sample type
Cell culture supernatant, Milk, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasma
Assay type
Sandwich (quantitative)
Sensitivity
20.6 pg/ml
Range
125 pg/ml -8000 pg/ml

Intra-assay
Sample	n	Mean	SD	CV%
Serum	3			5%

Inter-assay
Sample	n	Mean	SD	CV%
Serum	5			8.3%

Recovery

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 102 90%- 117%
Milk 102 91%- 111%
Serum 93 87%- 102%
Cell culture extracts 89 80%- 105%
Tissue Extracts 90 80%- 131%
EDTA Plasma 86 81%- 94%
Cit plasma 99 87%- 120%

Assay time
1h30m
Assay duration
One step assay
Species reactivity
Reacts with:Human, Rhesus monkeyDoes not react with:Cow
Product overview
Human Fibronectin ELISA Kit (ab219046) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, cit plasma, edta plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibronectin with 20.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITYThis kit recognizes both native and recombinant human Fibronectin protein in serum, plasma (citrate and EDTA), milk, cell culture supernatant, and cell and tissue extract samples only.
CROSS REACTIVITY Recombinant human Anastelin was prepared within the working range of the assay and assayed for cross reactivity. No cross-reactivity was observed.SPECIES REACTIVITYThis kit recognizes human Fibronectin protein.
Other species reactivity was determined by measuring 1/100,000 serum (mouse, rat, cow) or plasma (Rhesus macaque) samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution.
Notes
Fibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis and nerve regeneration. Differential expression of fibronectin is seen in coronary heart disease, glomerulopathy and tumor cell metastasis. The protein contains binding sites for collagen, heparin and fibrin and is a specific ligand for several integrin adhesion receptors. Fibronectin exists as a dimer or multimer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)

Sample specific recovery
Sample type	Average %	Range
Cell culture supernatant	102	90%- 117%
Milk	102	91%- 111%
Serum	93	87%- 102%
Cell culture extracts	89	80%- 105%
Tissue Extracts	90	80%- 131%
EDTA Plasma	86	81%- 94%
Cit plasma	99	87%- 120%

Storage instructions
Store at +4°C. Please refer to protocols.

Components	1 x 96 tests
10X Wash Buffer PT (ab206977)	1 x 20ml
50X Cell Extraction Enhancer Solution (ab193971)	1 x 1ml
5X Cell Extraction Buffer PTR (ab193970)	1 x 10ml
Antibody Diluent 4BR	1 x 6ml
10X Human Fibronectin Capture Antibody	1 x 600µl
10X Human Fibronectin Detector Antibody	1 x 600µl
Human Fibronectin Lyophilized Recombinant Protein	2 vials
Plate Seals	1 unit
Sample Diluent NS (ab193972)	1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit
Stop Solution	1 x 12ml
TMBDevelopment Solution	1 x 12ml

Research areas
- Cardiovascular
- Angiogenesis
- Adhesion / ECM
- Extracellular Matrix
- Signal Transduction
- Cytoskeleton / ECM
- Extracellular Matrix
- ECM Proteins
- Fibronectin
- Stem Cells
- Lineage Markers
- Ectoderm
- Stem Cells
- Neural Stem Cells
- Extracellular
- Stem Cells
- Mesenchymal Stem Cells
- Osteogenesis
- Cancer
- Invasion/microenvironment
- ECM
- Extracellular matrix
- Other
- Developmental Biology
- Lineage specification
- Ectoderm
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Involvement in disease
Glomerulopathy with fibronectin deposits 2
Sequence similarities
Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 16 fibronectin type-III domains.
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
Post-translationalmodifications
Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Cellular localization
Secreted, extracellular space, extracellular matrix.
Target information above from: UniProt accessionP02751The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).
Information by UniProt
Alternative names
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
- DKFZp686F10164
- DKFZp686H0342
- DKFZp686I1370
- DKFZp686O13149
- ED B
- Fibronectin 1
- FINC
- FINC_HUMAN
- FN
- FN1
- FNZ
- GFND
- GFND2
- LETS
- Migration stimulating factor
- MSF
- Ugl-Y3
see all
Database links
- Entrez Gene:2335 Human
- Omim:135600 Human
- SwissProt:P02751 Human
- Unigene:203717 Human

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Abcam/Human Fibronectin ELISA Kit (ab219046)/1 x 96 tests/ab219046

Overview

Product name

Detection method

Precision

Sample type

Assay type

Sensitivity

Range

Recovery

Assay time

Assay duration

Species reactivity

Product overview

Notes

Platform

Properties

Storage instructions

Research areas

Function

Tissue specificity

Involvement in disease

Sequence similarities

Developmental stage

Post-translationalmodifications

Cellular localization

Alternative names

Database links