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| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Human serum | 8 | 7% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Human serum | 3 | 9% |
| Sample type | Average % | Range |
|---|---|---|
| Serum | 109 | 105%- 112% |
| Cell culture media | 99 | 93%- 102% |
| Hep Plasma | 110 | 104%- 112% |
| Cit plasma | 115 | 107%- 123% |
Human GLP1 (7-36) ELISA kit (ab184857) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GLP-1(7-36) active peptide protein in human serum, plasma and culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GLP1 with25 pg/mL sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
GLP-1 (Glucagon like peptide 1) is part of the group of incretin hormones that are secreted by the gastrointestinal tract in response to food intake to assist glucose stimulated insulin secretion and glucagon suppression. GLP-1 is a 30 aminoacid peptide cleaved from proglucagon and released by the L-cells of the distal ileum. The intracellular precursor of GLP-1 (1-37) is cleaved to form the active peptides GLP-1 (7-37) and GLP-1 (7-36)NH2. The active peptides bind to the GLP-1 receptor (GLP-1r) expressed in the pancreatic beta cell and are quickly metabolized by the enzyme dipeptidyl peptidase IV (DPP-IV) to form the peptide GLP-1 (9-36), which has no insulin stimulating activity. Binding of active GLP-1 to the receptor, increases cAMP levels and potentiates insulin secretion via Protein Kinase A (PKA) and the cAMP-regulated nucleotide exchange factor (Epac2).GLP-1 and its receptor are also suggested to play a role in the central nervous systems as mediators of satiety. Intracerebroventricular GLP-1 has been shown to induce c-FOS activity in the hypothalamus and the central nucleus of the amygdala, both of which are important in the regulation of appetite.
| Components | 1 x 96 tests |
|---|---|
| 10X Human GLP1 (7-36) Capture Antibody | 1 x 600µl |
| 10X Human GLP1 (7-36) Detector Antibody | 1 x 600µl |
| 10X Wash Buffer PT (ab206977) | 1 x 20ml |
| Antibody DiluentCPI - HAMA Blocker (ab193969) | 1 x 6ml |
| Human GLP1 Lyophilized Recombinant Protein | 2 vials |
| Plate Seals | 1 unit |
| Sample Diluent NS (ab193972) | 1 x 50ml |
| SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
| Stop Solution | 1 x 12ml |
| TMBDevelopment Solution | 1 x 12ml |
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