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| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Serum | 5 | 3.3% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Serum | 3 | 4.8% |
| Sample type | Average % | Range |
|---|---|---|
| Cell culture supernatant | 107 | 104%- 113% |
| Serum | 108 | 97%- 117% |
| Hep Plasma | 105 | 97%- 117% |
| EDTA Plasma | 94 | 85%- 103% |
| Cit plasma | 97 | 86%- 110% |
Human Hemopexin ELISA Kit (ab221838) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemopexin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemopexin with 370 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer"s disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.
| Components | 1 x 96 tests |
|---|---|
| 10X Human Hemopexin Capture Antibody | 1 x 600µl |
| 10X Human Hemopexin Detector Antibody | 1 x 600µl |
| Human Hemopexin Lyophilized Native Protein | 1 x 2 vials |
| Antibody Diluent 4BI | 1 x 6ml |
| 10X Wash Buffer PT (ab206977) | 1 x 20ml |
| TMBDevelopment Solution | 1 x 12ml |
| Stop Solution | 1 x 12ml |
| Sample Diluent NS (ab193972) | 1 x 50ml |
| SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
| Plate Seals | 1 unit |
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